白藜芦醇对兔实验性骨关节炎血液流变学的影响

Effect of Resveratrol on Blood Rheology of Osteoarthritis in Rabbits

关键词:  骨关节炎  白藜芦醇  血液流变学  兔

Key words:  Osteoarthritis  resveratrol  blood rheology  rabbit

摘要:

目的探讨白藜芦醇对骨关节炎(osteoarthritis OA)模型兔血液流变学的影响。方法42只健康新西兰兔分为正常对照组、假手术组、模型组、阳性药物组、白藜芦醇高、中、低剂量组，每组6只。除正常对照组和假手术组外，均用Hulth法建立实验性骨关节炎模型。造模8周后分别以10%羧甲基纤维素钠(2 mL·kg-1)、阳性药物硫酸氨基葡萄糖(35 mg·kg-1)、白藜芦醇高剂量(120 mg·kg-1·d-1)、中剂量(60 mg·kg-1·d-1)、低剂量(30 mg·kg-1·d-1)灌胃，连续给药6周后，抽血测定血液流变学全血黏度、血浆黏度、相对黏度和聚集指数指标并作统计学处理。结果模型组的全血黏度、血浆黏度、相对黏度和聚集指数指标均高于正常对照组和假手术组(P<0.05)。而阳性药物组、白藜芦醇组全血黏度、血浆黏度、相对黏度和聚集指数指标均低于模型组 (P<0.05)，白藜芦醇高、中剂量组与低剂量组之间比较有统计学意义(P<0.05)，与正常对照组和假手术组比较无统计学意义(P>0.05)。结论白藜芦醇能显著降低骨关节炎模型兔全血黏度、血浆黏度、相对黏度和聚集指数指标，有效改善骨关节炎血液流变学状态，从而起到防止并延缓关节软骨的退行性改变，有利于软骨的修复作用。

Abstract:

OBJECTIVE To study the effect of resveratrol on blood rheology in the serum of osteoarthritis(OA) in rabbits. METHODS 42 New Zealand white rabbits were randomly collected 12 as the normal control group and the sham group. Other rabbits are construct the osteoarthritis model rabbit above the models of doing by Hulth. After 8 weeks of operation, we take the model rabits into 5 groups: model groups, positive medicament control group(glucosamine sulphate, 35 mg·kg-1), the low dosage group(resveratrol, 30 mg·kg-1·d-1), the middle dosage group(resveratrol, 60 mg·kg-1·d-1), the high dosage group(resveratrol, 120 mg·kg-1·d-1). There are 6 rabbits in each groups. Then the normal control group, sham group and model groups were received stomach irritation daily with 10% CMC-Na (2 mL·kg-1) and the positive medicament control group was received stomach irritation daily with glucosamine sulphate (35 mg·kg-1). The resveratrol low, middle, high dosage group were received stomach irritation daily with resveratrol in different dosage for 6 weeks (30 mg·kg-1, 60 mg·kg-1, 120 mg·kg-1). After 6 weeks, blood samples(blood viscosity, plasma viscosity, opposite viscosity, index of red blood cell aggregation) were determined and the data was used to make statistical analysis. RESULTS The determination of hemorrheologic (blood viscosity, plasma viscosity, opposite viscosity, index of red blood cell aggregation) of the model group was higher than that of the normal group and the sham group (P<0.05) . The determination of hemorrheologic of the resveratrol groups and the positive control group were lower than that of the model group(P<0.05). The high, middle dosage group were lower than that of the low dosage group. However, there were no significant differences between the high, middle dosage group and the normal group, sham group (P>0.05). CONCLUSION Resveratrol could reduce the expression of blood viscosity, plasma viscosity, opposite viscosity, index of red blood cell aggregation in osteoarthritis model rabbits. Resveratrol might effectively improve the blood rheology condition of osteoarthritis, preventing and delaying the degenerative changes in the articular cartilage.