HPLC和微生物法测定万古霉素的血药浓度

Comparison between HPLC and Microbiological Method for Determination of the Content of Vancomycin in Human Serum

关键词:  万古霉素  高效液相色谱法  微生物法  血药浓度

Key words:  vancomycin  HPLC  microbiological assay  serum drug concentration

摘要:

目的比较HPLC和微生物法测定万古霉素血药浓度的差异。方法30名病人静脉滴注万古霉素1.0 g，1次·d-1，分别用HPLC和微生物法测定用药前后的血药浓度。结果HPLC法在5.0～80 μg·mL-1浓度内线性关系良好，回收率97.3%～101.6%，日内、日间RSD分别为1.74%～4.06%和1.22%～4.21%；微生物法线性范围在5.0～60 μg·mL-1，回收率：98.2%～100.8%，日内、日间RSD分别为4.22%～5.51%和4.59%～6.49%。结论2种测定方法回收率和精密度均符合要求，测得的血药浓度无显著性差异。

Abstract:

OBJECTIVE To compare HPLC and microbiological assay for determination of the content of vancomycin in human serum. METHODS Thirty patients were given 1.0 g vancomycin by infusion，q.d..The serum concentration were determined by HPLC and bioassay，respectively. RESULTS The calibration curve of HPLC was within the range of 5.0-80.0 μg·mL-1. The recovery of HPLC method was 97.3% to 101.6%，within-day and day-to-day RSD were 1.74%-4.06%，and 1.22% -4.21%, respectively. The calibration curve of microbiological assay was linear in the range of 5.0-60.0 μg·mL-1. The recovery of microbiological assay was 98.2%-100.8%，within-day and day-to-day RSD were 4.22%-5.51% and 4.59%-6.49%，respectively. CONCLUSION The recovery and precision of the both methods are similar. There were no significant differences.