大规模提取罗汉果叶和藤及其多种生物活性的研究

Multi-functional Study of Extract Isolated from Leaf and Vine of Lo Han Kuo (Siraitia grosvenori Swingle) in Large-scale Extraction

关键词:  

Key words:  Momordica grosvenori  amberlite chromatography  antibacterial activity  antioxidant activity  insulin- stimulating activity

摘要:

目的 大规模制备罗汉果叶和藤提取物并研究其抗菌、抗氧化和促进胰岛素分泌的多种活性。方法 制备罗汉果叶和藤提取物，并通过离子交换树脂进一步分离。通过活性测试对提取物和各馏分进行抗菌、抗氧化和促进胰岛素分泌的试验。结果 罗汉果叶和藤提取物以及分离得到的馏分表现出抗菌、抗氧化和促进胰岛素分泌的活性。结论 罗汉果叶和藤提取物具有抗菌、抗氧化和促进胰岛素分泌的活性。罗汉果叶和藤具有开发为药物和功能性食品的潜力。

Abstract:

OBJECTIVE This research intended to isolate and compare the antibacterial and antioxidant activities of Lo Han Kuo plant (Siraitia grosvenori Swingle) by extracting and purifying bioactive substances from the leaf/vine of Lo Han Kuo plant in large-scale purification. METHODS Extracts were prepared from Lo Han Kuo leaf/vine, and the extracts were further purified by large-scale amberlite column. The antibacterial, antioxidant, and insulin-stimulating activities of these extracts were tested and compared. RESULTS The Lo Han Kuo leaf/vine parts exhibited antibacterial, antioxidant, and insulin-stimulating activities. To further isolate the bioactive fractions, the extracts were purified with amberlite chromatography and the purified fractions were tested again for the bioactivities. These bioactive fractions are located in different amberlite fractions. CONCLUSION The experimental data demonstrated that extracts of Lo Han Kuo leaves and vines exhibited antibacterial, antioxidant and insulin-stimulating activities. Lo Han Kuo leaf/vine appears to have the potential to be used for pharmaceutical preparations and dietary supplements to treat various human ailments.