HPLC测定鲜肿节风挥发油中b-榄香烯的含量

Determination of β-elemene in Volatile Oil of Fresh Sarcandra Glabra (Thunb.) Nakai by HPLC

关键词:  鲜肿节风挥发油  b-榄香烯  含量测定

Key words:  volatile oil of fresh Sarcandra glabra (Thunb.) Nakai  β-elemene  determination

摘要:

目的 建立鲜肿节风挥发油中b-榄香烯的TLC鉴别方法和HPLC含量测定方法。方法 TLC鉴别采用硅胶G薄层板，以正己烷为展开剂；HPLC含量测定采用Waters 5C₁₈-AR-Ⅱ (4.6 mm×250 mm，5 μm) 色谱柱，甲醇-乙腈-水为流动相，流速1 mL·min^-1，检测波长210 nm，柱温30 ℃。结果 供试品薄层色谱在与β-榄香烯色谱相应的位置上，显相同的紫红色斑点。b-榄香烯在0.221 5~2.214 8 μg呈良好的线性关系(r=0.999 9)，精密度试验的 RSD为1.89%，方法平均回收率为98.49%，其RSD为1.02%(n=6)。结论 该方法为评价鲜肿节风挥发油的质量提供了可靠保证。

Abstract:

OBJECTIVE To develop methods for identification and determination of β-elemene in volatile oil of fresh Sarcandra glabra (Thunb.) Nakai by TLC and HPLC. METHODS N-hexane was used as developing agent in TLC identification. The HPLC was carried out with a Waters Alliance, Model 2695, equipped with an Waters 5C₁₈-AR-Ⅱ column(4.6 mm×250 mm, 5 μm). It was eluted gradiently at 30 ℃ with a mixture of methanol-acetonitrile-water at flow-rate 1 mL·min^-1, and detection wavelength was set at 210 nm. RESULTS The same prunosus spots appeared at the corresponding place in the sample and β-elemene TLC. The relationship of β-elemene injection amount and peak area was linear (r=0.999 9) over the range of 0.221-52.214 8 μg. The RSD of accurate experiment was 1.89%. The average recovery was 98.49%( RSD 1.02%, n=6). CONCLUTION The method could be used for quality control of the volatile oil of fresh Sarcandra glabra (Thunb.) Nakai.