乌贼墨提取物对氧化性损伤小鼠心肌与脑组织的保护作用研究

Study of Sepia Ink Extract on Protection from Oxidative Damage of Cardiac Muscle and Brain Tissue in Mice

关键词:  乌贼墨提取物  氧化性损伤  心肌  脑组织

Key words:  squid ink extract  oxidative damage  cardiac muscle  brain tissue

摘要:

目的 探讨乌贼墨提取物对氧化性损伤小鼠心、脑组织的保护作用以及在不同剂量条件下的作用效果。方法 40只昆明系健康小鼠随机分为空白对照组、模型组、低、高剂量组。模型组腹腔注射环磷酰胺造氧化性损伤模型。检测各组小鼠心肌和脑组织中SOD(超氧化物歧化酶)，MDA(丙二醛)，CAT(过氧化氢酶)，GSH-Px(谷胱甘肽过氧化物酶)变化，血液中SOD，MDA变化。结果 环磷酰胺均能不同程度的影响上述指标的变化(P<0.01或P<0.05)，说明造模成功。与模型组比较，不同剂量的乌贼墨提取物均能不同程度的抑制由环磷酰胺所致的上述指标的变化(P<0.01或P<0.05)，但部分数据表明，较高浓度的提取物的抑制作用有所减弱，有的甚至不起作用。 结论 适当浓度的乌贼墨提取物对氧化性损伤小鼠心肌和脑组织具有一定的保护作用。

Abstract:

OBJECTIVE To explore the protective effects of sepia ink extract on oxidative damage of cardiac muscle and brain tissue in mice. METHODS Mice were randomly divided into 4 groups: control group, model group, low-dose group and high-dose group. Model animals were established with abdominal injection of cyclophosphamide. Contents of MDA, SOD, CAT and GSH-Px in cardiac muscle and brain tissue and contents of MDA, SOD in sera were determined. RESULTS Cyclophosphamide incoordinately changed the contents of MDA, SOD, CAT and GSH-Px in cardiac muscle, brain tissue and the contents of MDA, SOD in sera(P<0.01 or P<0.05). Compared with model group, sepia ink extract at suitable concentration could ameliorate significantly the changes of parameters induced by cyclophosphamide (P<0.01 or P<0.05), whereas, the high concentration of sepia ink extract had no obvious effect on the changes. CONCLUSION Sepia ink extract at certain concentration can protect cardiac muscle and brain tissue in mice from oxidative damage induced by cyclophosphamide.