4-氨基水杨酸钠结肠靶向微丸对溃疡性结肠炎大鼠免疫机制影响

Effect of 4-Amino Salicylic Acid Colon Specific Pellet on Ulcerative Colitis Rat Model of Immune Mechanism

关键词:  

Key words:  sodium 4-amino salicylic acid  the colon-specific pellet  ulcerative colitis  IL-1B  TNF-a  superoxide dismutase  malondialdehyde  myeloperoxidase

摘要:

目的 观察 4-氨基水杨酸钠(4-ASANa)结肠靶向微丸对溃疡性结肠炎大鼠的干预作用及其机制。方法 采用三硝基苯磺酸(TNBS)法制备大鼠实验性溃疡性结肠炎模型，通过4-ASANa结肠靶向微丸干预14 d，观察大鼠结肠大体形态损伤、组织学变化和白细胞介素-1B(IL-1B)、肿瘤坏死因子-α(TNF-α)、超氧化物歧化酶(SOD)、丙二醛(MDA)、髓过氧化物酶(MPO)等指标的变化。结果 4-ASANa结肠靶向微丸能减轻溃疡性结肠炎的病理损伤，显著降低溃疡性结肠炎大鼠血清IL-1B和TNF-a含量，差异具有统计学意义(P<0.05)，降低结肠组织中MDA和MPO含量，而提高SOD活性，差异具有统计学意义(P<0.05)，对化学因素引起的结肠炎有较好的治疗作用。结论 4-ASANa结肠靶向微丸对TNBS诱导的大鼠结肠炎具有治疗作用，抑制IL-1B和TNF-a的增殖和表达，减轻自由基的损害可能是作用机制之一。

Abstract:

OBJECTIVE To investigate effects and mechanism of sodium 4-amino salicylic acid colon localization pellet on chronic ulcerative colonitis. METHODS The rat ulcerative colonmitis model was made with TNBS. With 14 days of treatment through the pellets. The pathology of colon, IL-1B, TNF-a, superoxide dismutase (SOD) and malondialdehyde (MDA) were measured in all groups. RESULTS The sodium 4-amino salicylic acid colon localization pellet could alleviate pathologic damage of ulcer ative colonitis; debased IL-1B, TNF-a in serum, myeloperoxidase (MPO) and MDA in colon, increased SOD in colon; better to cure ulcrrative colonitis arosed by chemicals. CONCLUSION The sodium 4-amino salicylic acid colon localization pellet can effectively inhibit the inflammatory infiltration, inhibit IL-1B and TNF-a expression of the proliferation and to reduce free radical damage may be one of the mechanisms play a role.