芦荟凝胶通过上调bFGF和下调ICAM-1的表达防治大鼠多柔比星外渗性损伤

Aloe Gel Prevents and Cures Doxorubicin-Induced Extravasation Injury in Rats by Up-Regulating Expression of bFGF and Down-Regulating Expression of ICAM-1

关键词:  芦荟凝胶  多柔比星  外渗性损伤  bFGF  ICAM-1

Key words:  aloe gel  doxorubicin  extravasation injury  bFGF  ICAM-1

摘要:

目的 探讨芦荟凝胶对多柔比星外渗性损伤的防治机制。方法 建立SD大鼠多柔比星外渗性损伤动物模型，将70只SD大鼠随机分组：未损伤对照组、假手术组、多柔比星外渗性损伤组、溶媒对照组、芦荟凝胶低浓度保护组(芦荟凝胶0.1 g·L^-1)、芦荟凝胶中浓度胶保护组(芦荟凝胶1.0 g·L^-1)和芦荟凝胶高浓度保护组(芦荟凝胶10.0 g·L^-1)。测量外渗性损伤的面积以观察损伤程度，HE染色观察病理形态学改变，免疫组化检测损伤部位的皮肤及皮下组织中的bFGF、ICAM-1表达和分布情况。结果 不同浓度的芦荟凝胶保护组的外渗性损伤面积均小于多柔比星外渗性损伤组，且呈浓度依赖性(P<0.05)。HE染色结果显示：不同浓度的芦荟凝胶保护组大鼠外渗性损伤处其损伤程度轻于多柔比星外渗性损伤组。免疫组化检测结果显示：多柔比星外渗性损伤组与未损伤对照组比较，bFGF表达减少，ICAM-1表达增加。而不同浓度的芦荟凝胶保护组与多柔比星外渗性损伤组比较，bFGF表达递增，ICAM-1表达递减，且呈浓度依赖性(P<0.05)。结论 芦荟凝胶对大鼠多柔比星外渗性损伤有良好的防治作用，促进伤口愈合的机制可能与其促进bFGF的表达和抑制ICAM-1的表达有关。

Abstract:

OBJECTIVE To investigate the preventive effect of aloe gel on doxorubicin-induced extravasation injury and the underlying mechanism. METHODS Sprague-Dawley (SD) mice were used to establish the extravasation injury model induced by doxorubicin. Seventy SD mice were randomly divided into seven groups: non-injured control group, simulated operation group, doxorubicin-induced extravasation injured group, dissolvant control group, hyp-concentration (0.1 g·L^-1) aloe gel protected group, meta-concentration (1.0 g·L^-1) aloe gel protected group, hypsi-concentration (10.0 g·L^-1) aloe gel protected group. The areas of the extravasation injuries were measured to observe the degree of doxorubicin-induced extravasation injury. The pathological morphology was observed by optical microscopy. Expression of basic fibroblast growth factor (bFGF) and intercellular adhesion molecule (ICAM-1) in the exosmosis skin and subcutaneous tissues was detected by immunohistochemistry. RESULTS The areas of extravasation injuries in aloe gel protected groups at different concentration were smaller than that in doxorubicin-induced extravasation injured group (P<0.05). The result of HE stain revealed that the degree of extravasation injuries in aloe gel protected groups at different concentration was obviously less severe than that in doxorubicin-induced extravasation injured group. The result of immunohistochemistry revealed that the expression level of bFGF was lower and ICAM-1 was higher in doxorubicin-induced extravasation injured group than that in non-injured control group, while the expression level of bFGF was gradually higher and ICAM-1 was gradually lower in aloe gel protected groups at different concentration than that in doxorubicin-induced extravasation injured group, in a dose-dependent manner. CONCLUTION Aloe gel prevents doxorubicin-induced extravasation injury. Its mechanism is related with the up-regulation of bFGF expression and down-regulation of ICAM-1 expression.