罗红霉素分子印迹聚合物的合成及其特性研究

Synthesis and Evaluation of Molecularly Imprinting Polymer Using Roxithromycin as Template

关键词:  分子印迹聚合物  罗红霉素  吸附  功能单体

Key words:  molecularly imprinting polymer  roxithromycin  adsorption  functional monomer

摘要:

目的 探讨采用分子印迹技术合成罗红霉素分子印迹聚合物的方法，并对其特性进行研究。方法 以罗红霉素为模板分子，α-甲基丙烯酸为功能单体，以乙二醇二甲基丙烯酸酯为交联剂，采用分子印迹技术合成分子印迹聚合物。通过特异性吸附实验研究分子印迹聚合物的特性。结果 通过静态平衡吸附法研究了模板聚合物的结合动力学以及该聚合物的结合能力和选择特性，通过Scatchard分析法研究了印迹聚合物对模板分子的结合特性，经计算聚合物的最大表观结合量(Q_max)和平衡离解常数(Kd)分别为90.3 mg·g^-1和1.35 mg·mL^-1。结论 合成的分子印迹聚合物有形状、大小以及识别位点都与模板分子相匹配的空穴，即具有印迹作用。

Abstract:

OBJECTIVE To synthesize molecularly imprinting polymer(MIP) for roxithromycin by using molecular imprinting technique(MIT), and study the characterisation of it. METHODS A MIP with special molecule recognition properties of roxithromycin was prepared by thermal polymerization in which roxithromycin as template molecule, α-methacrylic acid as functional monomer and ethylene glycol dimethacrylate as the crosslinker. RESULTS Equilibrium binding experiment was used to investigate the adsorption dynamics, the binding ability to template molecule and the substrate selectivity. The Scatchard analysis was used to study that the MIP's binding characteristic to template molecule. By the analysis of Scatchard plot, the apparent maximum number (Q_max) and the equilib-rium dissociation constant (Kd) were found to be 90.3 mg·g^-1 and 1.35 mg·mL^-1, respectively. CONCLUSION The MIP for roxithromycin we synthsized produces a cavity with molecular recognition sites that can bind selectivity to the template, that is to say that it has imprinting effectiveness.