乙肝转阴散对鸭乙型肝炎病毒的抑制作用

Inhibitory Effect of Yiganzhuanyinsan on Duck Hepatitis B Virus

关键词:  乙肝转阴散  鸭乙型肝炎病毒  鸭乙型肝炎表面抗原  鸭乙型肝炎E抗原。

Key words:  Yiganzhuanyinsan  duck hepatitis B virus  DHBsAg  DHBeAg

摘要:

目的 观察乙肝转阴散(YGZYS)对鸭乙型肝炎病毒乙型肝炎表面抗原(DHBsAg)和乙型肝炎E抗原(DHBeAg)的抑制作用。方法 采用1 d龄雏鸭接种广西麻鸭乙型肝炎病毒(DHBV)强阳性血清，接种1周后用聚合酶链式反应(PCR)法筛选出DHBV强阳性鸭。随机分成5组：模型组、阳性对照组和YGZYS高、中、低剂量组，每组10只，各组雏鸭均灌胃给药14 d。于用药前(T0)、用药7 d (T7)和14 d (T14)及停药后3 d (P3)分别采血，采用酶联免疫吸附(ELISA)法测定血清DHBsAg和DHBeAg的滴度。结果 与模型组比较，YGZYS高、中剂量组用药后血清DHBsAg和DHBeAg的滴度显著降低(P<0.01或P<0.05)；停药3 d后，YGZYS高、中剂量组血清DHBsAg和DHBeAg的滴度无反跳现象。YGZYS抑制DHBsAg和DHBeAg的作用有明显的量效和时效反应关系。结论 YGZYS可有效地抑制DHBsAg和DHBeAg的表达。

Abstract:

OBJECTIVE To study the inhibitory effects of traditional Chinese medicine Yiganzhuanyinsan (YGZYS) on hepatitis B surface antigen (DHBsAg) and hepatitis B e-antigen (DHBeAg). METHODS One-day old Guangxi brown spotted ducks infected with hepatitis B virus (DHBV) were used as the DHBV infected animal model. Positive ducks were detected by PCR at one week after the infection of DHBV, and were randomly divided into five groups: the high dose group, middle dose group and low dose group of Yiganzhuanyinsan(YGZYS), model group and positive control group, with 10 ducks in each group. YGZYS was given ig. for 14 days. Serum samples were collected on the 0, 7 th, 14 th day respectively after the treatment with drugs and the 3rd day after drug withdrawal, and the contents of DHBsAg and DHBeAg in serum were measured by ELISA. RESULTS The serum DHBsAg and DHBeAg contents in high dose and middle dose groups of YGZYS were decreased significantly (P<0.01 or P<0.05), and did not rebound on the 3rd day after drug withdrawal, and its inhibitory effects on DHBsAg and DHBeAg were an obvious dose-and time-dependent manners. CONCLUSION YGZYS can effectively inhibit the expressions of DHBsAg and DHBeAg.