HPLC测定千柏鼻炎片中对羟基桂皮酸和金丝桃苷的含量

Simultaneous Determination of p-Hydroxycinnamic Acid and Hyperoside in Qianbai Biyan Tablets by HPLC

关键词:  千柏鼻炎片  千里光  对羟基桂皮酸  金丝桃苷

Key words:  Qianbai Biyan tablets  herba Senecionis Scandentis   p-hydroxycinnamic acid  hyperoside

摘要:

目的 建立千柏鼻炎片中对羟基桂皮酸和金丝桃苷的含量测定方法。 方法 应用高效液相色谱法测定，色谱柱为Dikma Diamonsil-C₁₈(4.6 mm×250 mm，5 µm)；流动相为乙腈-0.2%冰醋酸(15∶85)；流速1.0 mL·min^-1；检测波长310 nm (0~25 min)，360 nm (25~35 min)。 结果 对羟基桂皮酸和金丝桃苷的线性范围分别为0.014 088~1.174 µg (r=0.999 9)，0.009 55~1.91 µg (r=0.999 9)；平均加样回收率分别为101.17%(RSD=1.35%)，100.89% (RSD=3.80%)。 结论 该方法简便易行、准确、重复性好，可用于千柏鼻炎片的质量控制。

Abstract:

OBJECTIVE To establish a high performance liquid chromatographic method for the determination of p-hydroxycinnamic acid and hyperoside in Qianbai Biyan tablets. METHODS The chromatographic separation was achieved on a Dikma Diamonsil-C₁₈(250 mm×4.6 mm, 5 µm) column at a variable wavelength from 310 nm (0-25 min) to 360 nm (25-35 min) using an isocratic mobile phase consisted of a mixture of 0.2% aqueous acetic acid and acetonitrile in the ratio of 85∶15 at a flow rate of 1.0 mL·min^-1. RESULTS Linearity relationships were 0.014 088-1.174 µg (r=0.999 9)，0.009 55-1.91 µg (r=0.999 9), respectively; the average recoveries were 101.17%(RSD=1.35%) and 100.89% (RSD=3.80%). CONCLUSION The results showed that the method is simple, accurate and repeatable and it is suitable for the determination of p-hydroxycinnamic acid and hyperoside in Qianbai Biyan tablets.