维药阿里红多糖的提取及免疫活性研究

Studies on Extraction and the Immunity Activity of Polysaccharide from Fomes officinalis Ames

关键词:  阿里红  多糖  提取  含量测定  免疫活性

Key words:  Fomes officinalis Ames  polysaccharide  extraction  content determination  immuity activity

摘要:

目的 对新疆维药阿里红中的多糖进行提取，并研究阿里红粗多糖体外免疫活性。方法 建立热水提取、乙醇沉淀、Sevag法除蛋白的方法提取阿里红多糖(FoP)，红外及紫外吸收光谱检测其性质，采用苯酚-硫酸法测定多糖含量；采用MTT法检测不同浓度FoP在体外对小鼠脾细胞及腹腔巨嗜细胞增殖率的影响。结果 FoP产率为0.85%，多糖含量为68.81%；IR谱中，3 423，2 929，1 651，1 456，1 130，1 091，617 cm^-1处表现为典型的多糖吸收峰；UV谱中，260，280 nm处均无吸收峰；体外活性试验中，FoP对小鼠脾细胞、腹腔巨嗜细胞的生长具有明显的促进作用。结论 FoP具有一定的免疫调节活性。

Abstract:

OBJECTIVE To extract polysaccharide from Fomes officinalis Ames of Xinjiang and evaluate its immunity activity in vitro. METHODS Crude polysaccharide was extracted with hot water, precipitated with 95% ethanol, removed protein with Sevag method. Polysaccharide content was determined with phenol-sulfuric acid method. It was identified by IR and UV scanning spectrum. MTT method was applied to determine the immunity activities of the polysaccharide from Fomes officinalis Ames(FoP). RESULTS The yield of crude water-extracted polysaccharide was 0.85%. The polysaccharide content was 68.81%. IR spectrum indicated that there were typical characteristic absorption peaks of polysaccharide. UV scanning spectrum showed that there were no absorption peaks of protein and nucleic acid at point 280 nm and 260 nm. FoP increased the proliferation of mice spleen lymphocytes and peritoneal macrophages cell populations in vitro. CONCLUSION FoP has certain immunity activity.