北青龙衣中萘醌类衍生物的细胞毒活性研究

Studies on the Cytotoxicity of Naphthoquinone Derivatives from the Fresh Rejuvenated Fruits of Juglans mandshurica

关键词:  北青龙衣  萘醌类衍生物  细胞毒

Key words:  the fresh rejuvenated fruits of J. mandshurica  naphthoquinone derivatives  cytotoxicity

摘要:

目的 研究北青龙衣中萘醌类衍生物的细胞毒活性。方法 利用MTT法测定从北青龙衣中分离得到的12个萘醌类衍生物对SMMC7721人肝癌细胞和MCF-7人乳腺癌细胞的细胞毒活性。结果 12个萘醌类衍生物中，4-羟基萘-1-O-b-D-吡喃葡萄糖苷(9)，1,4,8-三羟基萘-1-O-b-D-吡喃葡萄糖苷(10)和1,4,8-三羟基萘-1-O-b-D-[6’-O-(3’’,4’’,5’’-三羟基苯甲酰)]吡喃葡萄糖苷(11)对SMMC7721人肝癌细胞显示不同程度的抑制作用，仅11对MCF-7人乳腺癌细胞显示较强的细胞毒作用。结论 北青龙衣中萘醌类衍生物在其抗肿瘤活性中可能起重要作用。

Abstract:

OBJECTIVE To study the cytotoxicity of naphthoquinone derivatives isolated from the fresh rejuvenated fruits of J. mandshurica. METHODS The cytotoxicity of 12 naphthoquinone derivatives were estimated by MTT assay against human liver SMMC 7721cancer cells and human breast MCF-7 cancer cells. RESULTS Among the 12 naphthoquinone derivatives, 4-hydroxynaphthalene-1-O-b-D-glucopyranoside (9), 1,4,8-trihydroxynaphthalene-1-O-b-D-glucopyra-noside (10) and 1,4,8- trihydroxynaphthalene-1-O-b-D-[6’-O-(3’’,4’’,5’’-trihydroxybenzoyl)] glucopyranoside (11) exhibited cytotoxiciy against human liver SMMC 7721 cancer cells whereas only 11 showed cytotoxicity against human breast MCF-7 cancer cells. CONCLUSION Naphthoquinone derivatives in this plant maybe play a significant role in its antitumor activities.