颐和春口服液对肾阳虚大鼠肾损伤的保护作用

The Protective Effects of Yihechun Oral Solution on Kidney of Kidney-YANG Deficiency Rats

关键词:  颐和春  肾阳虚  肾功能  抗凋亡  抗氧化

Key words:  Yihechun oral solution  deficiency of kidney-YANG  renal function  anti-apoptotic  antioxidant

摘要:

目的 观察颐和春口服液对肾阳虚大鼠肾功能的保护作用，并初步探讨其机制。方法 用腺嘌呤灌胃制备肾阳虚大鼠模型。实验分为正常对照组、肾阳虚模型组、肾阳虚阳性药物治疗组和肾阳虚颐和春治疗组。给药10 d后观察颐和春口服液对肾阳虚大鼠体重、肾指数、肾功能及肾形态学改变，并从凋亡和抗氧化方面探讨其保护机制。HE染色观察肾形态学改变、生化法测定血清谷胱甘肽过氧化物酶(GSH-PX)、血尿素氮(BUN)和肌酐(Cr)，末端标记法测定肾组织凋亡。结果 颐和春可改善肾阳虚引起的大鼠体重下降，使肾指数增加，降低血清肌酐(Cr)和尿素氮(BUN)水平，改善肾形态学表现，减轻肾小管上皮细胞凋亡，提高血清谷胱甘肽过氧化酶(GSH-PX)活性。结论 颐和春口服液对肾阳虚大鼠肾功能有明显的保护作用，可能与提高抗凋亡和抗氧化能力有关。

Abstract:

OBJECTIVE To observe the protective effect of Yihechun oral solution on the deficiency of kidney-YANG rats and to investigate its initial mechanism. METHODS Prepare deficiency of kidney-YANG rats model with intra-gastric adenine. Experimental groups were divided into normal control group, model group, positive drug treatment group and Yihechun treatment group. Observed the effect of Yihechun on the rats’ weight, renal index, renal function and kidney morphosis, and investigated its protection mechanism on the apoptosis and antioxygen. HE staining of renal morphological changes, biochemical serum GSH-PX, BUN, and Cr, end-labeling method for the determination of apoptosis in renal tissue. RESULTS Yihechun can improve weight loss in rats caused by deficiency to increased kidney index, lower serum creatinine (Cr) and blood urea nitrogen (BUN) level, to improve the performance of renal morphology and reduce apoptosis of renal tubular epithelial cells, raise serum glutathione peroxidase (GSH-PX) activity. CONCLUSION Yihechun oral solution has significantly protective effects on the renal function in deficiency of kidney-YANG rats. Its mechanism may be associated with increased resistance to apoptosis and the antioxidant capacity .