魟鱼肝脏鱼油的提纯及EPA和DHA的检测

Purification of Ray Fish Liver Oil and Determination of EPA and DHA

关键词:  魟鱼肝脏  鱼油  EPA  DHA  高效液相色谱法

Key words:  ray liver  fish oil  eicosapentaenoic acid  docosahexaenoic acid  HPLC

摘要:

目的 研究从魟鱼肝脏中提取、精炼、纯化鱼油的方法，并测定其EPA和DHA含量。方法 采用正己烷超声提取，磷酸脱胶，氢氧化钠脱酸，活性碳脱色，旋转蒸发脱臭和脱腥，盐析法提取总脂肪酸，尿素乙醇包合获得高纯度的不饱和脂肪酸，然后用HPLC测定 EPA和DHA含量。结果 精炼后的鱼油为浅黄色澄清透明，有略淡的鱼腥味，其各项指标均达到了SC/T3502-2000标准的一级要求；纯化后的鱼油质量为肝脏质量的16.1%，其中EPA和DHA的含量分别为19.54%和57.62%。结论 魟鱼肝脏中提取、精炼、纯化鱼油的方法可行，检测方法可靠。

Abstract:

OBJECTIVE To establish the method of extraction, refinement and purification for fish oil from ray fish liver, and to develop a analytical method for the determination of the content of EPA and DHA. METHODS Highly purfied unsaturated fatty acids were purified by N-hexane ultrasound extraction, H₃PO₄ degumming, NaOH depickling, active carbon decolor, rotary evaporation deodorization, salt fractionation extraction total fatty acids, urea complexation. The content of EPA and DHA was detected by HPLC. RESULTS The results showed that refined fish oil was yellow, transparent with light traing flavour and each index reached the requirement of SC/T3502-2000. The total mass fraction of refined fish oil in liver was up to 16.1%, and the content of EPA and DHA was 19.54% and 57.62%, respectively. CONCLUSION This is a feasible method for extraction, refinement and purification of fish oil in the liver of the ray. The method for detection is reliability.