左归丸对免疫性卵巢早衰小鼠性激素的影响

Influence of Zuogui Pill on Sex Hormone in Peripheral Blood Serum of Immune Premature Ovarian Failure Rats

关键词:  左归丸  卵巢早衰  性激素

Key words:  Zuogui pill  premature ovarian failure  sex hormone

摘要:

目的 观察左归丸对免疫性卵巢早衰(POF)小鼠外周血抗透明带抗体(AZpAb)、FSH、E₂水平变化的影响，进一步揭示中药补肾养血活血法在调节卵巢功能方面的作用机制。方法 以小鼠透明带3所合成透明带多肽为免疫原，免疫SPF级BALB/C雌性小鼠，建立小鼠免疫性POF模型，随机分为模型组、左归丸高、中、低剂量组，强的松组、己烯雌酚组和对照组，共灌胃给药4周。采用ELISA法检测外周血FSH及E₂、AZpAb值。结果 FSH、AZpAb组间的比较测定值都有显著性差异；各用药组之间对正常组及模型组比较：己烯雌酚组的FSH值有极显著的意义，左归丸高、低剂量组的E2值有显著的意义，左归丸高、中、低剂量组及己烯雌酚组的AZpAb值都有极显著的意义。结论 左归丸能使阴虚小鼠外周血中雌二醇含量增加，减缓POF小鼠外周血FSH的升高，抑制AZpAb产生，改善POF小鼠卵巢免疫性炎症的损伤。

Abstract:

OBJECTIVE To explore the effect of Zuogui pill (ZGP) , a Chinese compound recipe for tonifying Shen, on AZpAb，FSH, E₂ in peripheral blood serum of immune premature ovarian failure (POF) rats and to further reveal the mechanism in regulating ovarian function in rats. METHODS BALB/C grade SPF female mice model of POF was established by multiple sites subcutaneous injection of zona pellucida 3 of mice, and treated with different dosage ZGP of low, middle and high one for 4 weeks, with prednisone and diethylstilbestrol as positive control. Detected AZpAb，FSH, E₂ in peripheral blood serum by ELISA . RESULTS There was significantly different within groups FSH and AZpAb, and the FSH was significantly different in diaethylstilbestrol group, the E2 value was significantly different in ZGP of low and high dosage group, and it markedly different in AZpAb value in ZGP of low and high dosage and diaethylstilbestrol groups. CONCLUSION Zuogui pill may improve the immuno-inflammation damage in ovaries of POF rat by increasing the concentration of E2 value in peripheral blood and inhibiting AZpAb production.