脂联素对内皮祖细胞氧化损伤的保护作用及机制研究

Protective Effects of Adiponectin on Oxidative Damage of EPCs and Its Possible Mechanism

关键词:  脂联素  内皮祖细胞  氧化低密度脂蛋白  氧化损伤

Key words:  adiponectin  endothelial progenitor cells  oxidative low density lipoprotein  oxidative damage

摘要:

目的　观察脂联素对氧化低密度脂蛋白(oxidative low density lipoprotein，Ox-LDL)诱导的人外周血内皮祖细胞(endothelial progenitor cells，EPCs)氧化损伤的保护作用，并探讨其可能的机制。方法 密度梯度离心法获取外周血单个核细胞，通过流式细胞仪鉴定内皮祖细胞。培养7 d后，收集贴壁细胞并随机分为正常对照组、Ox-LDL组(10 µg·mL^-1)及脂联素干预组(Ox-LDL 10 µg·mL^-1加脂联素，浓度分别为1.25，2.5，5 µg·mL^-1)，干预 24 h后采用细胞活力噻唑蓝(MTT)法测定细胞活力。并取各组细胞上清液行超氧化物歧化酶(SOD)、丙二醛(MDA)含量检测。结果 脂联素可以改善Ox-LDL诱导氧化损伤的EPCs的活力，增加SOD活性，降低MDA含量。结论 脂联素对氧化损伤的EPCs具有保护作用，其机制可能与提高抗氧化酶活性有关。

Abstract:

OBJECTIVE To observe the protective effects of adiponectin on oxidative low density lipoprotein(Ox-LDL)- induced oxidative damage of endothelial progenitor cells (EPCs) from peripheral blood. And study its possible mechanism. METHODS Total mononuclear cells(MNCs) were isolated from peripheral blood by Ficoll density gradient centrifugation, and were identified by demonstrating the expression of CD34, VEGFR-2 and AC133 with flow cytometry, to make sure that all the cells needed were EPCs. Then the cells were plated on fibronectin-coated culture dishes. After incubation for 7 days, attached cells were collected and divided into three groups: control group, Ox-LDL group(10 µg·mL^-1), adiponectin intervention group, stimulated with different cencentrations of adiponectin (1.25, 2.5, 5 µg·mL^-1). The cells viability was examined by methyl thiazoiyl tetrazolium(MTT) assay after intervented for 24 h. And cell supernate of each group was carried on the SOD, MDA content examination. RESULTS In Ox-LDL group, the cells viability and SOD content obviously droped, the MDA content obviously elevated. The cells viability and SOD content of adiponectin intervention group obviously increased and the MDA content obviously reduced. CONCLUSION Adiponectin has protective effects on the oxidative injured EPCs induced by Ox-LDL. Its mechanism may be related to the function of anti-oxidase activity.