| 引用本文: | 刘丽娟,王常禹,麻风华.北青龙衣细胞毒活性部位中的萘酚苷[J].中国现代应用药学,2010,27(8):704-708. |
| .Naphthalenyl Glucosides from the Cytotoxic Portion of the Fresh Rejuvenated Fruits of Juglans Mandshurica[J].Chin J Mod Appl Pharm(中国现代应用药学),2010,27(8):704-708. |
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| 摘要: |
| 目的 研究北青龙衣细胞毒活性部位中的化学成分。方法 利用MTT法测定北青龙衣醇提取物经大孔树脂柱色谱得到的4个组分对胃癌细胞BGC803的细胞毒作用，对其中活性最强的30%乙醇洗脱部位经硅胶柱色谱和反相硅胶柱色谱进行化学成分的分离。结果 得到4个萘酚苷类化合物并经核磁共振谱确定了它们的结构，分别为1,4,5-三羟基萘-1,4-二-O-β-D-吡喃葡萄糖苷(1)，1,4,5-三羟基萘-1,5-二-O-β-D-吡喃葡萄糖苷(2), 1,4,8-三羟基萘-1-O-β-D-吡喃葡萄糖苷(3)，1,4,8-三羟基萘-1-O-β-D-[6′-O-(3″,4″,5″-三羟基苯甲酰)] 吡喃葡萄糖苷(4)。结论 北青龙衣细胞毒活性部位中含有萘酚苷类化合物，可能在其抗肿瘤活性中起重要作用。 |
| 关键词: 北青龙衣 细胞毒 萘酚苷 |
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| Naphthalenyl Glucosides from the Cytotoxic Portion of the Fresh Rejuvenated Fruits of Juglans Mandshurica |
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LIU Lijuan1 WANG Changyu2 MA Fenghua2
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| Abstract: |
| OBJECTIVE To study the chemical components of the cytotoxic portion of the fresh rejuvenated fruits of Juglans mandshurica. METHODS The cytotoxicity of four elutes from macro-porous resin chromatography was estimated by MTT assay against human stomach BGC803 cancer cells. The 30% ethanol elute showing the strongest activities was isolated by silica gel chromatography and reverse phase silica gel chromatography. RESULTS 1,4,5-trihydroxynaphthalene-1,4- di-O-β-D-glucopyranoside(1), 1,4,5-trihydroxynaphthalene-1,5-di-O-β-D-glucopyranoside(2), 1,4,8-trihydroxynaphthalene-1-O-β-D-glucopyranoside(3), 1,4,8-trihydroxynaphthalene-1-O-β-D-[6′-O-(3″,4″,5″-trihydroxybenzoyl)] glucopyranoside(4) were isolated and their structures were determined by NMR spectrum. CONCLUSION There are naphthalenyl glucosides in the cytotoxic portion of fresh rejuvenated fruits of Juglans mandshurica and they may play a significant role in its antitumor activities. |
| Key words: the fresh rejuvenated fruits of Juglans mandshurica cytotoxicity naphthalenyl glucosides |
