九里香叶总黄酮-羟丙基-β-环糊精包合物的制备、鉴定和热力学稳定性研究

Preparation, Identification and Thermodynamic Stability Study of Murraya Exotica L. Leaves Flavonoids Hydroxypropyl-b-Cyclodextrin Inclusion Complex

关键词:  九里香  包合物  差示扫描量热法  X射线衍射法  相溶解度法

Key words:  Murraya exotica L.  inclusion complex  differential scanning calorimetry  X-ray diffraction  phase solubility

摘要:

目的 制备和鉴定九里香叶总黄酮-羟丙基-β-环糊精包合物，并考察九里香叶总黄酮和羟丙基-β-环糊精之间的包合摩尔比及包合过程的热力学常数。方法 采用溶液-搅拌法制备九里香叶总黄酮-羟丙基-β-环糊精包合物，采用差示扫描量热法、X射线衍射法和红外光谱法对包合物进行鉴定，通过表观溶解度法考察包合物中主客分子之间的包合摩尔比及包合过程的热力学常数。结果 25，35，45 ℃时九里香叶总黄酮和羟丙基-β-环糊精能形成1∶1 摩尔比包合物，相溶解度图呈AL型，包合过程为放热反应。结论 九里香叶总黄酮与羟丙基-β-环糊精能自发地形成1∶1 摩尔比包合物，从而显著提高九里香叶总黄酮在水中的溶解度。

Abstract:

OBJECTIVE To prepare and identify Murraya exotica L. leaves flavonoids hydroxypropyl-β-cyclodextrin inclusion complex(JK-HP-β-CD). The molar ratio between Murraya exotica L. leaves flavonoids and HP-β-CD as well as the thermodynamic constants in inclusion was also studied. METHODS The JK-HP-β-CD inclusion complex was prepared with solution-agitating method. Meanwhile, the inclusion complex was identified by differential scanning calorimetry, X-ray diffraction and infrared spectrometry, respectively. The molar ratio between host and guest molecules and the thermodynamic constants during the inclusion process were also investigated by phase solubility method. RESULTS A 1∶1 molar ratio inclusion complex of JK with HP-β-CD could be formed at 25, 35, 45℃. The phase diagram was AL type and the procedure of inclusion was a heat release process. CONCLUSION A 1∶1 molar ratio inclusion complex of JK with HP-β-CD could be formed spontaneously, which increased the solubility of JK in aqueous solution obviously.