HPLC测定不同产地枫树叶中没食子酸的含量

Determination of Gallic Acid in Leaf of Liquidambar taiwaniana Hance in Different Areas by HPLC

关键词:  高效液相色谱法  枫树叶  没食子酸

Key words:  HPLC  leaf of Liquidambar taiwaniana Hance  gallic acid

摘要:

目的 建立高效液相色谱法测定不同产地枫树叶中没食子酸的含量。方法 色谱柱为Alltima C₁₈(4.6 mm×250 mm，5 µm)；流动相为乙腈-0.05%磷酸溶液(4∶96)；流速为1.0 mL·min^-1；检测波长为270 nm；柱温为30 ℃。结果 没食子酸进样浓度在0.019 08～0.229 0 mg·mL^-1(r=0.999 6)内与峰面积呈良好的线性关系，平均回收率为101.52%，RSD为1.40%(n=6)。结论 该方法简便、准确、重复性好，可用于枫树叶的质量控制。

Abstract:

OBJECTIVE To establish the method of determination of gallic acid in Leaf of Liquidambar taiwaniana Hance in different areas. METHODS The chromatographic conditions included Alltima C₁₈ column(4.6 mm×250 mm, 5 μm) and the mobile phase consisted of acetonitril-0.05% phosphoric acid(4∶96). Detection wavelength was at 270 nm. The flow rate was 1.0 mL·min^-1. Column temperature was at 30 ℃. RESULTS There was a good linear within the range of 0.019 08-0.229 0 mg·mL^-1 for Gallic acid with r=0.999 6. The average recovery was 101.52% with RSD=1.40%(n=6). CONCLUDION The method is simple, accurate, reproducible and can be used for the quality control of Liquidambar taiwaniana Hance.