GC-MS和化学计量学解析法分析白术中挥发油成分

Analysis of Essential Oil in Rhizoma Atractylodes Macrocephala by GC-MS and Chemometric Resolution Method

关键词:  白术  挥发油  气相色谱-质谱  化学计量学解析法

Key words:  Rhizoma Atractylodes Macrocepha  essential oil  GC-MS  chemometric resolution method

摘要:

目的 分析白术挥发油中的化学成分。方法 采用气相色谱-质谱(GC-MS)联用技术对其进行分离检测，利用化学计量学解析法(CRM)对重叠色谱峰进行分辨解析，并结合程序升温保留指数辅助定性，从而对挥发油成分进行准确的定性定量分析。结果 共分辨出了33个色谱峰，鉴定了其中29个组分，占白术挥发油总量的95.93%，其中主要化学成分为2-(2-甲氧基)苯甲氧基苯酚、γ-芹子烯和3,7(11)-蛇床二烯等，它们占总挥发油的70.07%，而其他26个组分只占25.86%。结论 结合使用CRM解析重叠色谱峰，比单独使用GC-MS能更真实、全面地反映白术中的挥发油化学成分。

Abstract:

OBJECTIVE To analyze the essential oil components in Rhizoma Atractylodes Macrocephala. METHODS Gas chromatography-mass spectrometry (GC-MS) combined with chemometric resolution method(CRM), a useful chemometric resolution method which can be used to resolve overlapping peaks in chromatogram, together with the temperature-programmed retention index for assistant identification, was employed to qualitative and quantitative analysis of the essential oil components. RESULTS A total of 33 chromatographic peaks were resolved, and 29 components in the essential oils were identified, which accounted for 95.93% of the total relative contents. The main chemical compositions in the studied sample were 2-(2-methoxyphenoxy)phenol, γ-selinene and 3,7(11)-selinadiene, etc. All of these volatile alcohols accounted for about 70.07% of the total essential oils, and the other 26 constituents only accounted for about 25.86% of the total contents. CONCLUSION It could more really and comprehensively reflect the essential oil components in Rhizoma Atractylodes Macrocepha by GC-MS combined with CRM method, which can be used to resolve the overlapping chromatographic peaks than just used the GC-MS.