隐丹参酮对APP/PS1双转基因模型小鼠学习记忆和抗氧化能力的影响

Influence of Cryptotanshinone on Memory and Antioxidation Ability in APP/PS1 Double Transgenic Mice

关键词:  隐丹参酮  阿尔茨海默病  学习记忆  氧化应激

Key words:  cryptotanshinone  Alzheimer’s disease  learning and memory  oxidative stress

摘要:

目的 本实验利用APP/PS1双转基因模型小鼠，观察隐丹参酮对小鼠行为学习及抗氧化能力的影响。方法 选用APP/PS1双转基因模型小鼠，将其随机为5组，另取同窝阴性小鼠作为正常对照组，每组10只，3个月龄后开始给药。给药4个月后，通过行为学实验测试小鼠学习记忆能力的变化；采用分光光度法测定血清和脑组织中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)的含量。结果 Morris水迷宫实验显示，APP/PS1双转基因模型小鼠逃避潜伏期较正常组明显延长，原象限停留时间明显缩短。隐丹参酮组小鼠逃避潜伏期较模型组明显缩短，原象限停留时间明显延长。APP/PS1双转基因模型小鼠血清和脑组织GSH-Px、SOD活力下降，MDA含量明显增多；隐丹参酮治疗组学习和记忆能力升高，血清和脑组织GSH-Px、SOD活力升高，MDA含量减少，与阿尔茨海默病模型组比较有显著性差异。结论 APP/PS1双转基因模型小鼠7个月后发展有明显学习记忆功能障碍伴有抗氧化能力的下降，隐丹参酮对阿尔茨海默病模型小鼠学习记忆障碍具有明显改善作用并且可剂量依赖性的显著改善阿尔茨海默病模型小鼠的抗氧化能力。

Abstract:

OBJECTIVE To study the effect of cryptotanshinone on memory and antioxidation ability in APP/PS1 double transgenic mice. METHODS Three-month-old APP/PS1 double transgenic mice were randomly divided into 5 gorups. Besides, negative control mice were served as control group. There were 10 mice in each group and all of them were intragastrically administered for 4 months. Then the learning and memory ability of mice were detected by Morris water maze test. The activities of glutathione peroxidase(GSH-Px), superoxide dismutase(SOD) and the content of malondialdehyde(MDA) in serum and brain tissue were measured with spectrophotometer. RESULTS Compared with control group, APP/PS1 double transgenic mice significantly prolonged the latency of searching the hidden platform in directional swimming test, decreased the time spent in swimming in the target quadrant in probe test. Compared with APP/PS1 double transgenic mice, cryptotanshinone could dose-dependently shorten the latency of searching the hidden platform in directional swimming test, increased the time spent in swimming in the target quadrant in probe test. The activities of GSH-Px and SOD decreased and the MDA content increased in APP/PS1 double transgenic mice, while in cryptotanshinone treatment groups, the activities of GSH-Px and SOD heightened and MDA content decreased, and there was significant difference compared with Alzheimer’s disease model group. CONCLUSION Seven-month-old APP/PS1 double transgenic mice became imitated learning and memory dysfunction and the antioxidation ability reduction; cryptotanshinone could dose-dependently ameliorate the impaired learning and memory function of Alzheimer’s disease mice and improve antioxidation ability.