氯沙坦联合小剂量叶酸对自发性高血压大鼠血液流变学和血管结构的影响

Effect of Losartan with Low Dose Folic Acid on Improving Hemorheology and Vascular Endothelium in Spontaneous Hypertensive Rats

关键词:  氯沙坦  叶酸  自发性高血压大鼠  血液流变学  血管

Key words:  losartan  Folic acid  spontaneously hypertensive rats  hemorheology  vascular

摘要:

目的 评价氯沙坦联合小剂量叶酸对自发性高血压大鼠(SHR)血液流变学和血管结构的影响。方法 30只12周龄SHR，随机分为3组(n=10)，空白对照组(SHR-C)，氯沙坦组(SHR-L：50 mg·kg^-1·d^-1)，合用组(SHR-L+Y：氯沙坦25 mg·kg^-1·d^-1 +叶酸0.4 mg·kg^-1·d^-1)，另选10只同龄Wistar大鼠作为正常对照组(WKY-C)。连续给药12周后，测定血液流变学指标，采用单因素方差分析，以LSD法进行组间比较。肠系膜血管组织切片用HE染色，主动脉测定血管壁厚度和管腔面积，计算厚度和管腔面积。结果 SHR-L+Y组与SHR-C相比，全血黏滞度各指标均下降，P<0.05；SHR-L+Y组与SHR-L相比，全血黏滞度无明显差别，P>0.05。血浆黏滞度，各组间差别不显著，P>0.05。血管组织学观察显示：SHR-L+Y组肠系膜动脉无增厚，内皮细胞层完整，内弹性膜结构清晰，主动脉中膜面积和管腔面积比与SHR-C组相比有统计学差异，P<0.05。结论 低剂量的氯沙坦和叶酸能有效的改善血液流变学，保护血管内皮，防止血管重构。

Abstract:

OBJECTIVE To investigate the efficacy of losartan-Folic acid in hemorheology level and vascular endothelium. METHODS Thirty spontaneously hypertensive rats(SHR), aged 12 weeks, enrolled for a randomized controlled trial. Subjects were randomly assigned to the following three groups(n=10)：controlled group (SHR-C), losartan group(SHR-L: 50 mg·kg^-1·d^-1), losartan-Folic group (SHR-L+Y: losartan 25 mg·kg^-1·d^-1+Folic acid 0.4 mg·kg^-1·d^-1), another 10 of the same age Wistar Rats assigned for normal controlled group(WKY-C). Hemorheology was examined after oral administration for 12 weeks. The data was performed analysis of variance and LSD-t. Vessels of mesentery and aorta were stained by HE. The thickness and area of aorta was measured and analyzed by computer. RESULTS The viscosity of whole blood was decreased significantly in SHR-L+Y group compared with SHR-C, P<0.05.There are no obvious difference of hemorheology between SHR-L+Y group and SHR-L group, P>0.05. Effects on plasma viscosity was not significantly different among the four groups. For morphological remodeling in group SHR-L+Y, there are no thickness increased in mesenteric artery and endothelial wall is integrated, aortic tunica intima is smooth, the media cross-sectional area and vessel area of SHR-L+Y compared with group SHR-C was not significantly different, P<0.05. CONCLUSION Losartan-Folic acid could be used to decrease the viscosity, protect vascular endothelium and reversing vascular reconstruction.