响应面法优化地参多糖提取工艺及其抗氧化活性分析

Optimization of Polysaccharides Extraction from Lycopus lucidus Turcz. by Response Surface Methodology and Study on Its Antioxidant Activity

关键词:  地参  多糖  纤维素酶  超声提取  响应面法  抗氧化性

Key words:  Lycopus lucidus Turcz.  polysaccharides  cellulose  ultrasonic extraction  response surface methodology  antioxidant properties

摘要:

目的 优化纤维素酶协同超声辅助提取地参多糖的工艺参数，并分析其抗氧化性。方法 在单因素试验基础上，以液料比（A）、纤维素酶用量（B）、超声功率（C）、超声温度（D）为自变量，以地参多糖提取量（Y）为响应值，采用4因素4水平的响应面分析法优化地参多糖提取工艺，同时测定地参多糖对DPPH自由基的清除能力。结果 纤维素酶协同超声辅助提取多糖的最佳工艺条件为：液料比60 mL·g^-1、纤维素酶用量3.6%、超声功率300 W、超声温度30℃、超声时间40 min，在此条件下，地参多糖提取量为（378.56±1.346）mg·g^-1，与预测值（381.68 mg·g^-1）接近。地参多糖对DPPH自由基有较强的清除能力，且随多糖质量浓度的增加而逐渐增强；地参多糖质量浓度为0.420 mg·mL^-1时，清除率为91.36%。结论 采用响应曲法优化的多糖提取工艺参数准确可靠，可用于地参多糖提取；地参多糖具有较强的体外抗氧化能力。

Abstract:

OBJECTIVE To optimize the technology parameters of polysaccharides extraction from Lycopus lucidus Turcz. by cellulase and ultrasound, and its antioxidant activity is analyzed. METHODS On the basis of single factor experiment, with liquid to material ratio(A), cellulase dosage(B), ultrasonic power(C) and ultrasonic temperature(D) as independent variables, and the extraction rate(Y) of Lycopus lucidus Turcz. polysaccharides as response value, the extraction process of polysaccharides from Lycopus lucidus Turcz. was optimized by response surface analysis with 4 factors and 4 levels, at the same time, the scavenging capacity of Lycopus lucidus Turcz. polysaccharides to DPPH free radical was also determined. RESULTS The optimum conditions for the extraction were:liquid to material ratio 60 mL·g^-1, cellulase dosage 3.6%, ultrasonic power 300 W, ultrasonic temperature 30℃, ultrasonic time 40 min, under these conditions, the extraction amount of polysaccharides from Lycopus lucidus Turcz. was (378.56±1.346)mg·g^-1, which was close to the predicted value(381.68 mg·g^-1). Lycopus lucidus Turcz. polysaccharides had a certain scavenging effect on DPPH free radical, and gradually increased with the increase of polysaccharides concentration. When the concentration of polysaccharides was 0.420 mg·mL^-1, the scavenging rate was 91.36%. CONCLUSION The parameters of polysaccharides extraction process optimized by response surface method are accurate and reliable, which can be used for the extraction of Lycopus lucidus Turcz. polysaccharides. Lycopus lucidus Turcz. polysaccharides has strong antioxidant capacity in vitro.