星点设计-响应面法优化密闭微波辅助提取辣椒素工艺

Optimization of Pressurized Microwave-assisted Extraction Technology of Capsaicin by Central Composite Design-response Surface Methodology

关键词:  辣椒素  星点设计  密闭微波提取  响应面法  辣椒

Key words:  capsaicin  central composite design  pressurized microwave-assisted extraction  response surface  Gapsici Fructus

摘要:

目的 优化密闭微波辅助提取辣椒素的提取工艺。方法 以提取率为因变量，以粒度、液料比、提取时间、提取温度为考察单因素，采用星点设计-响应面优化法优化提取工艺条件，通过方差分析及线性、二项式回归方程模型拟合，进行预测分析。结果 经优化得到最佳实验条件为药材粒度90目，液料比（mL：g）43：1，微波温度85℃，微波时间18 min，微波功率为550 W，75%乙醇做提取溶剂。在此条件下，辣椒素得率为7.59 mg·g^-1（RSD=2.52%，n=3），与模型预测值相符。结论 与传统溶剂回流法相比，该方法简单、提取率更高。

Abstract:

OBJECTIVE To optimize extraction technology of capsaicin by pressurized microwave-assisted extraction. METHODS Using extraction rate as dependent variables, using particle size, ratio of liquid to solid, extraction time, extraction temperature as independent variables, central composite design-response surface methodology were used to optimize the extracting process conditions. Through variance analysis and linear and binomial regression equation model fitting, the prediction analysis was carried out. RESULTS The optimum extraction conditions were as follows:particle size was 90 mesh, ratio of liquid to solid(mL:g) was 43:1, microwave temperature was 85℃, microwave time was 18 min, microwave power was 550 W, solvent was 75% ethanol. Under these conditions, the yield of capsaicin could reach 7.59 mg·g^-1(RSD was 2.52%, n=3), which was close to the model predictions. CONCLUSION Compared with traditional solvent reflux method, this method is simple and has higher extraction rate.