八月札抗肝癌活性部位的筛选

Screening of Anti-hepatocarcinoma Active Fractions from Akebiae Fructus

关键词:  八月札  有效部位筛选  抗肿瘤

Key words:  Akebiae Fructus  active fraction screening  anti-tumor

摘要:

目的 筛选出八月札抗肝癌的活性部位。方法 利用H22小鼠肝癌皮下移植瘤模型对八月札乙醇提取物及其石油醚萃取物、氯仿萃取物、乙酸乙酯萃取物、正丁醇萃取物和将乙醇提取后的药渣进行水提得到的水提物进行抗肝癌活性部位筛选，剂量均为生药20 g·kg^-1；采用人肝癌细胞BEL-7404裸鼠皮下移植瘤模型研究乙醇提取物的抗肝癌作用。结果 八月札乙醇提取物以及正丁醇萃取部位对H22小鼠肝癌皮下移植瘤模型具有明显的抑瘤作用，抑瘤率分别为46.87%和45.31%，其他部位无明显的抑瘤作用；八月札乙醇提取物的高剂量组（生药20 g·kg^-1）和中剂量组（生药10 g·kg^-1）对人肝癌细胞BEL-7404裸鼠移植瘤模型具有明显的抑瘤作用，抑瘤率分别为57.5%和40.0%。结论 乙醇提取物及其正丁醇萃取物部位为八月札抗肝癌活性部位。

Abstract:

OBJECTIVE To select anti-hepatocarcinoma effective parts of Akebiae Fructus. METHODS The H22 subcutaneously transplanted tumor in mice was applied to screen the anti-hepatocarcinoma active fractions from the ethanol extract, petroleum ether extract, chloroform extract, ethyl acetate extract, n-butanol extract and the water extract from residue extracted from ethanol, the dosage was crude 20 g drug·kg^‒1; the anti-hepatocarcinoma effect of ethanol extract on the nude mice model of human hepatoma line BEL-7404 cell subcutaneously transplanted tumor was studied. RESULTS The results showed that the ethanol extract and n-butanol extract of Akebiae Fructus had obvious anti-tumor effect on H22 mice model of subcutaneous transplantation of liver cancer, the inhibition rates were 46.87% and 45.31%, respectively. The other parts had no obvious antitumor effect; the high-dose group(crude drug 20 g·kg^‒1) and the medium dose group(crude drug 10 g·kg^‒1) of the ethanol extract of Akebiae Fructus had obvious antitumor effect on BEL-7404 transplanted tumor model in nude mice, and the inhibition rates were 57.5% and 40.0%, respectively. CONCLUSION The ethanol extract and its n-butanol extract are the antitumor active fractions of Akebiae Fructus.