灵芝孢子油对大鼠肝纤维化的干预作用

Antifibrotic Effect of Ganoderma Lucidum Spore Oil in Rats

关键词:  肝纤维化  灵芝孢子油  四氯化碳  氧化损伤

Key words:  liver fibrosis  Ganoderma lucidum spores oil  carbon tetrachloride  oxidative damage

摘要:

目的 探讨灵芝孢子油对大鼠肝纤维化的影响。方法 选择40% CCl₄皮下注射诱导SD大鼠肝纤维化形成，造模同时用低、中、高剂量（0.33，0.67，2.00 g·kg^-1）灵芝孢子油灌胃干预（每组10只），观察血浆谷草转氨酶（AST）、谷丙转氨酶（ALT）、丙二醛（MDA）、透明质酸（HA）和肝组织MDA、羟脯氨酸（HyP）等的变化，肝组织HE和Masson染色观察病理变化。结果 灵芝孢子油能显著降低CCl₄所致的肝纤维化大鼠血浆AST、ALT、MDA、HA水平（P<0.05），降低肝组织HyP、MDA含量（P<0.05），肝脏病理显示纤维化程度明显减轻（P<0.05）。结论 灵芝孢子油对CCl₄诱导的大鼠肝纤维化具有保护作用。

Abstract:

OBJECTIVE To investigate the effect of Ganoderma lucidum spore oil on liver fibrosis. METHODS Liver fibrosis was replicated by 40% CCl₄ injection subcutaneously in SD male rats. Meantime, three doses of Ganoderma lucidum spore oil low, medium and high(0.33, 0.67, 2.00 g·kg^-1) were used to intervene the formation of fibrosis by gavage (n=10, per group). Plasma activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA) and hyaluronic acid (HA), and the hepatic levels of MDA and hydroxyproline (HyP) were evaluated. Hematoxylin and eosin(HE) and Masson staining were used to observe the change of liver histology. RESULTS Plasma activities of AST, ALT, MDA and HA all were reduced significantly by Ganoderma lucidum spore oil in CCl₄ induced rats (P<0.05). Similar change was observed in hepatic content of Hyp and MDA(P<0.05), and further conformed by liver histology. CONCLUSION Ganoderma lucidum spore oil can alleviate liver fibrosis in rats caused by CCl₄.