原子吸收光谱法和原子荧光光谱法测定蜂产品中的重金属残留量

Determination of Heavy Metal Residues in Bee Products by Atomic Absorption Spectroscopy and Atomic Fluorescence Spectrometry

关键词:  原子吸收光谱法  原子荧光光谱法  蜂产品  铅  砷  汞

Key words:  graphite furnace atomic absorption spectrometry  atomic fluorescence spectrometry  bee products  Pb  As  Hg

摘要:

目的 建立不同种类蜂产品中铅、砷、汞的重金属残留量的检测方法。方法 采用微波消解法处理蜂产品，通过选择最佳消解条件和测定条件，利用石墨炉原子吸收光谱法测定蜂产品中铅含量，和利用原子荧光光谱法测定蜂产品中砷、汞含量，并对测定方法进行方法学考察。结果 铅、砷、汞测定方法线性关系分别为Y=0.003 04X+0.001 31（r=0.999 6）、Y=88.645 1X＋2.563 8（r=0.999 9）、Y=552.113 2X-6.169 5（r=0.999 6），RSD均<3%，回收率分别为98.2%，96.3%，93.8%，测定结果分别为0.003~4.203，0~0.192，0~0.097 6 mg·kg^-1。结论 不同种类蜂产品中重金属含量不同，其中铅含量最高，本方法适用于同时检测蜂产品中多种重金属残留。

Abstract:

OBJECTIVE To establish a method for determination of heavy metal residues of Pb, As and Hg in bee products. METHODS Samples were digested with microware digestion system, under the optimum digestive and experimental conditionds, Pb was determined by graphite furnace atomic absorption spectrometry, while As and Hg were determined by atomic fluorescence spectrometry, and the measurement methods were methodologically evaluated. RESULTS Calibration curves of 3 elements (Pb, As, Hg) were Y=0.003 04X+0.001 31(r=0.999 6), Y=88.645 1X+2.563 8 (r=0.999 9), Y=552.113 2X-6.169 5 (r=0.999 6), respectively. RSD were all <3%. The recovery values were 98.2%, 96.3%, 93.8%, respectively. The results of content determination were 0.003-4.203, 0-0.192, 0-0.097 6 mg·kg^-1, respectively. CONCLUSION The content of 3 kinds of heavy metals in bee products is different and Pb is the highest one. The method can be used for the determination of heavy metals in bee products.