| 引用本文: | 黄北雄,戴柏桉.ASE-HPLC测定保和丸中橙皮苷的含量[J].中国现代应用药学,2016,33(10):1304-1307. |
| HUANG Beixiong,DAI Baian.Determination of Hesperidin in Bohol Pill by ASE-HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(10):1304-1307. |
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| 摘要: |
| 目的 建立加速溶剂萃取（accelerated solvent extraction，ASE）-高效液相色谱法测定保和丸中橙皮苷含量的方法。方法 利用ASE提取保和丸中橙皮苷，采用HPLC测定保和丸中橙皮苷的含量，选用Kinete×2.6μXB-C18 100A色谱柱（100 mm×4.6mm，5 μm），流动相为甲醇-7%醋酸溶液（37：63），流速0.7 mL·min-1，柱温40℃，检测波长283 nm。结果 橙皮苷在3.707~74.140 μg·mL-1内与峰面积呈良好线性关系，平均加样回收率为99.33%，RSD为0.2%。结论 该方法快速、准确，可用于保和丸中橙皮苷含量的测定。 |
| 关键词: 加速溶剂萃取 高效液相色谱法 保和丸 橙皮苷 |
| DOI：10.13748/j.cnki.issn1007-7693.2016.10.020 |
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| Determination of Hesperidin in Bohol Pill by ASE-HPLC |
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HUANG Beixiong, DAI Baian
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Guangxi Wuzhou Institute for Food and Drug Control, Wuzhou 543001, China
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| Abstract: |
| OBJECTIVE To develop a method for the determination of hesperidin in Bohol pill by accelerated solvent extraction(ASE)-HPLC method. METHODS Hesperidin in Bohol pill was extracted by ASE. The analytical column was Kinetex XB-C18(100 mm×4.60 mm, 2.6 mm). The mobile phase was methanol-7% acetic acid(37:63) with flow rate 0.7 mL·min-1. The column temperature was 40℃. The detector wavelength was set at 283 nm. RESULTS The linear range was 3.707-74.140 μg·mL-1. The average recovery of hesperidin was 99.33%(RSD=0.2%). CONCLUSION The established method is simple, rapid and reliable, which can be applied to determine hesperidin in Bohol pill. |
| Key words: accelerated solvent extraction(ASE) HPLC Bohol pill hesperidin |
