| 引用本文: | 谭道鹏,杜艺玫,秦琳,鲁艳柳,张倩茹,何芋岐.HPLC测定大风艾中5种二氢黄酮类成分的含量[J].中国现代应用药学,2021,38(5):615-618. |
| TAN Daopeng,DU Yimei,QIN Lin,LU Yanliu,ZHANG Qianru,HE Yuqi.Simultaneous Determination of Five Flavanones in Blumea Balsamifera by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2021,38(5):615-618. |
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| HPLC测定大风艾中5种二氢黄酮类成分的含量 |
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谭道鹏1,2, 杜艺玫1, 秦琳1, 鲁艳柳1, 张倩茹1, 何芋岐1
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1.遵义医科大学药学院, 贵州遵义 563009;2.贵州医科大学省部共建药用植物功效与利用国家重点实验室, 贵阳 550014
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| 摘要: |
| 目的 测定大风艾药材中3，3'，5，7-四羟基-4'-甲氧基二氢黄酮、3'，5，5'，7-四羟基二氢黄酮、3，3'，4'，5-四羟基-7-甲氧基二氢黄酮、3，3'，5-三羟基-4'，7-二甲氧基二氢黄酮、艾纳香素5种二氢黄酮类成分的含量。方法 采用高效液相色谱法测定，色谱柱为AKZONOBEL Kromasil 100-5C18（4.6 mm×250 mm，5 μm）；流动相为乙腈（A）-0.2%冰醋酸（B），梯度洗脱（0~10 min，20% A； 10~15 min，20%→25% A； 15~25 min，25% A； 25~50 min，25%→65% A）；流速：1.0 mL·min-1；检测波长：289 nm；柱温：35℃；进样量：10 μL。结果 5种二氢黄酮类成分的线性关系良好，加样回收率为98.73%~101.84%。结论 该方法简便、准确，可用作大风艾药材质量控制的方法。 |
| 关键词: 大风艾 二氢黄酮 含量测定 |
| DOI：10.13748/j.cnki.issn1007-7693.2021.05.019 |
| 分类号:R917.101 |
| 基金项目:贵州医科大学药用植物功效利用国家重点实验室资助项目（FAMP201909K）；贵州省科技计划项目（黔科合平台人才[2017]5101，[2019]5657号）；贵州省中医药管理局中医药、民族医药科学技术研究项目（QZYY-2019-056） |
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| Simultaneous Determination of Five Flavanones in Blumea Balsamifera by HPLC |
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TAN Daopeng1,2, DU Yimei1, QIN Lin1, LU Yanliu1, ZHANG Qianru1, HE Yuqi1
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1.School of Pharmacy, Zunyi Medical University, Zunyi 563009, China;2.State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China
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| Abstract: |
| OBJECTIVE To determine content of 5 flavanones of 3,3',5,7- tetrahydroxy-4'-methoxyflavanone, 3',5,5',7- tetrahydroxy-flavanone, 3,3',4',5-tetrahydroxy-7-methoxyflavanone, 3,3',5-trihydroxy-4',7-dimethoxyflavanone, 5-blumeatin in Blumea balsamifera. METHODS The chromatographic separation was achieved on AKZONOBEL Kromasil 100-5C18 (4.6 mm×250 mm, 5 μm) column with the mobile phase consisting of acetonitrile(A)-0.2% HAc(B) by gradient elution program(0-10 min, 20%A; 10-15 min, 20%→25%A; 15-25 min, 25%A; 25-50 min, 25%→65%A) at the flow rate of 1.0 mL·min-1, detection wavelength was set at 289 nm, and the column temperature was 35℃, and the injection volume was 10 μL. RESULTS Linearity relationships of 5 flavanones were good, and the average recoveries were from 98.73% to 101.84%. CONCLUSION The method is simple, accurate and repeatable and it is suitable for quality control of Blumea balsamifera. |
| Key words: Blumea balsamifera flavanones content determination |
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