竹节参总皂苷通过HES1调节高脂饮食小鼠小肠上皮分泌型细胞数量的研究

Study on the Total Saponins of Panacis Japonici Rhizoma Regulating the Number of Secretory Cells of Small Intestinal Epithelium in Mice with High Fat Diet Through HES1

关键词:  竹节参总皂苷  高脂饮食  杯状细胞  潘氏细胞  HES1

Key words:  total saponins of Panacis Japonici Rhizoma  high fat diet  goblet cells  Paneth cells  HES1

摘要:

目的 探讨竹节参总皂苷对高脂饮食小鼠小肠上皮分泌型细胞数量的调节作用。方法 将40只Balb/c小鼠随机均分为4组，即对照组，高脂模型组和竹节参总皂苷高、低剂量组。对照组小鼠给予普通维持饲料，高脂模型组和竹节参总皂苷高、低剂量组小鼠均给予高脂饲料；另外，竹节参总皂苷高、低剂量组小鼠每天给予45，15 mg·kg^-1的竹节参总皂苷分别进行灌胃处理，高脂模型组和对照组以生理盐水进行灌胃，灌胃频次均为每天1次，每周6 d。各组小鼠均持续喂养7个月后处死，取材并进行HE染色观察各组高脂饮食小鼠回肠组织病理学改变，阿利新蓝染色、过碘酸-Schiff染色后统计小鼠回肠杯状细胞数量，荧光桃红酒石黄染色后统计小鼠潘氏细胞数量，免疫组化法检测小鼠回肠Lysozyme、HES1蛋白的表达。结果 与高脂模型组相比，竹节参总皂苷高剂量组小鼠回肠绒毛长度与隐窝深度比值、黏膜下层厚度显著降低（P < 0.05），内环肌层厚度显著增加（P < 0.01），Lysozyme蛋白表达、杯状细胞数量显著增加（P < 0.05或P < 0.001），HES1蛋白表达显著下调（P < 0.01）。结论 竹节参总皂苷通过抑制高脂饮食小鼠小肠HES1蛋白的表达调节小肠上皮分泌型细胞数量。

Abstract:

OBJECTIVE To investigate the mechanism of total saponins of Panacis Japonici Rhizoma on regulating the number of secretory cells of small intestinal epithelium in mice fed with high fat diet. METHODS Forty Balb/c mice were randomly divided into following 4 groups:control group, high-fat-diet model group, high and low dose of total saponins of Panacis Japonici Rhizoma groups. The mice of control group were given normal maintenance feed. Mice of high-fat-diet model group, high and low dose of total saponins of Panacis Japonici Rhizoma groups were given high-fat feed. In addition, mice of high and low dose groups were given 45, 15 mg·kg^-1 of total saponins of Panacis Japonici Rhizoma every day for oral gavage treatment respectively. Meanwhile, the high-fat diet model group and control group was given intragastric administration with normal saline. The frequency of gavage was to give gavage once a day for 6 d in a week. Mice of each group were sacrificed and ileum tissues were taken after feeding for 7 months. HE staining was used to observe ileum pathological changes of each group. Alcian blue staining, periodic acid-schiff staining were used to count ileum goblet cell of mice and fluorescein peach red staining was used to count Paneth cell number of mice. Immunohistochemical staining was used to detect protein expression levels of Lysozyme and HES1. RESULTS Compared with the high-fat-diet model group, high dose of total saponins of Panacis Japonici Rhizoma group decreased villus length-crypt depth ratio and submucosal layer thickness(P < 0.05), increased depth of crypt and inner ring muscle thickness(P < 0.01), increased goblet cell number and protein expression levels of Lysozyme(P < 0.05 or P < 0.001), and down-regulated protein expression levels of HES1(P < 0.01). CONCLUSION Total saponins of Panacis Japonici Rhizoma regulates the number of small intestinal secretory cells by inhibiting the expression of HES1 protein in the small intestine of mice fed with high fat diet.