| 引用本文: | 肖小武,刘静佳,周志强,许妍,罗跃华,付辉政,洪挺.GC-MS同时测定复方鲜竹沥液中8个酚类有效成分[J].中国现代应用药学,2022,39(6):788-793. |
| XIAO Xiaowu,LIU Jingjia,ZHOU Zhiqiang,XU Yan,LUO Yuehua,FU Huizheng,HONG Ting.Simultaneous Determination of 8 Phenolic Active Components in Fufang Xianzhuli Ye by GC-MS[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(6):788-793. |
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| GC-MS同时测定复方鲜竹沥液中8个酚类有效成分 |
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肖小武1, 刘静佳2, 周志强1, 许妍1, 罗跃华1, 付辉政1, 洪挺1
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1.江西省药品检验检测研究院, 国家药品监督管理局中成药质量评价重点实验室, 江西省药品与医疗器械质量工程技术研究中心, 南昌 330029;2.南昌理工学院, 南昌 330044
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| 摘要: |
| 目的 建立GC-MS同时测定复方鲜竹沥液中苯酚、愈创木酚、甲基愈创木酚、乙基愈创木酚、乙烯基愈创木酚、2,6-二甲氧基苯酚、香兰素和丁香醛8个酚类有效成分含量的方法。方法 样品经乙醚萃取后,采用DB-35MS UI色谱柱(30 m×0.25 mm,0.25 μm)进行分离,采用离子监测模式(SIM)进行质谱检测,外标法定量。结果 在优化实验条件下,8种酚类成分分离良好,在0.1~129.4 μg·mL-1内线性良好,相关系数均>0.999 0,方法检出限均<0.043 μg·mL-1;在3个不同加标浓度下的回收率为93.76%~101.33%,RSD值均≤ 3.08%。结论 该方法简单、灵敏、准确并且可靠,可为复方鲜竹沥液的质量控制提供科学依据。 |
| 关键词: 复方鲜竹沥液 酚类有效成分 气相色谱-质谱联用 |
| DOI：10.13748/j.cnki.issn1007-7693.2022.06.012 |
| 分类号:R284.2 |
| 基金项目:江西省重点研发计划项目(20203BBG73062);江西省药品监督管理局科研项目(2020JS04) |
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| Simultaneous Determination of 8 Phenolic Active Components in Fufang Xianzhuli Ye by GC-MS |
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XIAO Xiaowu1, LIU Jingjia2, ZHOU Zhiqiang1, XU Yan1, LUO Yuehua1, FU Huizheng1, HONG Ting1
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1.Jiangxi Provincial Institute for Drug Control, NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Medicine, Jiangxi Provincial Engineering Research Center for Drug and Medical Device Quality, Nanchang 330029, China;2.Nanchang Institute of Technology, Nanchang 330044, China
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| Abstract: |
| OBJECTIVE To establish a GC-MS method for the simultaneous determination of 8 phenolic active components such as phenol, guaiacol, 2-methoxy-4-methylphenol, 4-ethyl-2-methoxy-phenol, 4-hydroxy-3-methoxy-styrene, 2,6-dimethoxyphenol, vanillin and syringaldehyde in Fufang Xianzhuli Ye. METHODS The samples were extracted with ether and separated by a DB-35MS UI column(30 m×0.25 mm, 0.25 μm), then the extract was detected by MS under SIM mode. And external standard method was used for quantification. RESULTS Under the optimized experimental conditions, 8 phenolic components were well separated. The linearity was good within the mass concentration range of 0.1-129.4 μg·mL-1, correlation coefficients were >0.999 0. The detection limits of phenolic active components were <0.043 μg·mL-1. The spiked recoveries were in the range of 93.76%-101.33% with RSD ≤ 3.08%. CONCLUSION The method is simple, sensitive, accurate and reliable, and can provide some scientific basis for quality control of Fufang Xianzhuli Ye． |
| Key words: Fufang Xianzhuli Ye phenolic active components GC-MS |
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