| 引用本文: | 白璧炜,刘永成,岳云飞,王春贺,王常禹,孙晖.高效离子交换色谱法测定肌氨肽苷注射液中次黄嘌呤含量[J].中国现代应用药学,2022,39(3):339-342. |
| BAI Biwei,LIU Yongcheng,YUE Yunfei,WANG Chunhe,WANG Changyu,SUN Hui.Determination of Hypoxanthine in Muscular Amino Acids and Peptides and Nucleosides Injection by High Efficiency Ion Exchange Chromatography[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(3):339-342. |
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| 摘要: |
| 目的 建立采用磺酸基阳离子交换键合硅胶的离子交换色谱法，对肌氨肽苷注射液中次黄嘌呤含量进行测定。方法 采用磺酸基阳离子交换键合硅胶色谱柱；水（硫酸调节pH值至2.5±0.1）为流动相；二极管阵列检测器，检测波长为260 nm；流速0.8 mL·min-1；柱温30℃。结果 次黄嘌呤在1~50 μg·mL-1线性良好，平均回收率为99.0%。结论 该方法操作简便，色谱图基线稳定，主成分峰形对称，分离度高，方法的重复性和耐用性好、准确度高，可用于肌氨肽苷注射液中次黄嘌呤的含量测定。 |
| 关键词: 肌氨肽苷注射液 次黄嘌呤 离子交换色谱法 分离度 定量分析 |
| DOI：10.13748/j.cnki.issn1007-7693.2022.03.009 |
| 分类号:R917 |
| 基金项目:国家生化药标准提高项目（174） |
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| Determination of Hypoxanthine in Muscular Amino Acids and Peptides and Nucleosides Injection by High Efficiency Ion Exchange Chromatography |
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BAI Biwei1,2, LIU Yongcheng2, YUE Yunfei2, WANG Chunhe2, WANG Changyu2, SUN Hui1
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1.Research Center of Traditional Chinese Medicine Prescription Metabolomics, National Administration of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, China;2.Heilongjiang Institute for Drug Control, Harbin 150087, China
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| Abstract: |
| OBJECTIVE To establish a method for determining content of hypoxanthine in Muscular Amino Acids and Peptides and Nucleosides injection by ion used exchange chromatography of sulfonic cation exchange bonded silicone. METHODS The ion chromatographic column used exchange chromatography of sulfonic cation exchange bonded silicone were adopted, the mobile phase was water(pH adjusted to 2.5±0.1 with sulfuric acid) at a flow rate of 0.8 mL·min-1, the column temperature was 30℃, the photo-diode array detector was used with the detection wavelength of 260 nm. RESULTS There was a good linear relationship of hypoxanthine in the concentration of 1-50 μg·mL-1. The average recovery was 99.0%. CONCLUSION The method has the advantages of simple operation, stable chromatogram baseline, symmetrical principal component peaks, high resolution, good repeatability, durability and high accuracy. It can be used for the determination of hypoxanthine in Muscular Amino Acids and Peptides and Nucleosides injection |
| Key words: Muscular Amino Acids and Peptides and Nucleosides injection hypoxanthine ion exchange chromatography separation degree quantitative analysis |
