瑶药绣花针的质量标准研究

Study on Quality Standard of Yao Medicinal Damnacanthus Indicus (Linn.) Gaertn. F

关键词:  绣花针  虎刺醛  薄层色谱鉴别  高效液相色谱法  质量标准

Key words:  Damnacanthus indicus (Linn.) Gaertn. F.  damnacanfhal  TLC  HPLC  quality standard

摘要:

目的 建立瑶药绣花针质量标准，为该药材质量控制提供实验依据。方法 建立瑶药绣花针的性状、显微特征、薄层鉴别及含量测定方法；按照中国药典2020年版（第四部）通则中相关方法，对绣花针药材的水分、总灰分、酸不溶性灰分及浸出物等进行测定。结果 瑶药绣花针的性状、显微特征、薄层鉴别等均有明显的专属特征。瑶药绣花针药材含水量为7.4%~13.3%，总灰分为5.4%~9.6%，酸不溶性灰分为3.4%~7.8%，水溶性浸出物为13.3%~47.6%。虎刺醛浓度在20.6~144.2 μg·mL^–1（r²=0.999）内有良好的线性关系，平均加样回收率为98.38%。11批药材样品中虎刺醛含量在0.049%~0.107%，平均含量为0.085%。结论 建立的瑶药绣花针质量标准具有很好的专属性、稳定性和重现性，可用于瑶药绣花针药材的质量控制。

Abstract:

OBJECTIVE To establish the quality standard of Yao medicinal Damnacanthus indicus (Linn.) Gaertn. F. and to provide experimental basis for its quality control of the medicine. METHODS The identification of Yao medicinal properties, microscopic characteristics, TLC and content determination method of Damnacanthus indicus (Linn.) Gaertn. F. was conducted. The moisture, total ash, acid insoluble ash and extract of Damnacanthus indicus (Linn.) Gaertn. F. were determined according to the general principles of Chinese Pharmacopoeia 2020 Edition (Part IV). RESULTS Damnacanthus indicus (Linn.) Gaertn. F. under forest exhibited specific properties in characteristics, microscopic features and TLC results. The Yao medicinal Damnacanthus indicus (Linn.) Gaertn. F. moisture was 7.4%-13.3%, the total ash was 5.4%-9.6%, the acid insoluble ash was 3.4%-7.8%, and the water-soluble extract was 13.3%-47.6%. Damnacanthus Indicus (Linn.) Gaertn. F. had a good linear relationship in 20.6~144.2 μg·mL^-1(r²=0.999), and the average recovery rate was 98.38%. The content of damnacanfhal in 11 batches of medicinal materials ranged from 0.049% to 0.107%, with an average of 0.085%. CONCLUSION The established quality standard of Yao medicinal Damnacanthus indicu (Linn.) Gaertn. F. has good specificity, stability and reproducibility, which can be used for the quality control of Yao medicinal Damnacanthus indicus (Linn.) Gaertn. F..