环磷腺苷葡胺对人脐静脉血管内皮细胞增殖、迁移、管腔形成及凋亡的影响

Effect of Meglumine Cyclic Adenylate on Proliferation, Migration, Angiogenesis and Apoptosis in Human Umbilical Vein Endothelial Cells

关键词:  环磷腺苷葡胺  人脐静脉血管内皮细胞  增殖  血管生成  凋亡

Key words:  meglumine cyclic adenylate  human umbilical vein endothelial cells  proliferation  angiogenesis  apoptosis

摘要:

目的 探索环磷腺苷葡胺（meglumine cyclic adenylate，MCA）对人脐静脉血管内皮细胞（human umbilical vein endothelial cells，HUVEC）增殖、迁移、管腔形成及凋亡的影响。方法 通过显微镜观察、MTT法、划痕修复试验、管腔形成试验、Western blotting观察不同浓度MCA对HUVEC细胞形态、增殖、迁移、管腔形成能力及凋亡相关蛋白表达的影响。结果 显微镜观察发现，随着MCA剂量增加，HUVEC细胞形态由多角形变为卵圆形。MCA可呈浓度依赖地抑制HUVEC增殖、迁移、管腔形成，并且高剂量的MCA还可诱导细胞凋亡，表现为细胞凋亡数目增加，Bax、Cleaved-PARP蛋白表达上调，PARP、Bcl-2蛋白表达下调。结论 MCA可抑制HUVEC细胞增殖、迁移、管腔形成，并诱导细胞凋亡。

Abstract:

OBJECTIVE To explore the effects of meglumine cyclic adenylate(MCA) on the proliferation, migration, lumen formation and apoptosis of human umbilical vein endothelial cells(HUVEC). METHODS The effects of different concentrations of MCA on HUVEC cell morphology, proliferation, migration, luminal formation ability and apoptosis-related protein expression were observed by microscope observation, MTT method, scratch repair experiment, lumen formation experiment and Western blotting. RESULTS Microscopic observation revealed that with the increase of the dose of MCA, the morphology of HUVEC cells changed from polygonal to oval. MCA could inhibit HUVEC proliferation, migration and lumen formation in a concentration-dependent manner, and high-dose MCA could also induce cell apoptosis, which was manifested by an increase in the number of apoptosis, the expression of Bax, Cleaved-PARP protein was up-regulated and the expression of PARP and Bcl-2 protein was down-regulated. CONCLUSION Meglumine cyclic adenylate can inhibit HUVEC cell proliferation, migration, luminal formation and induce cell apoptosis.