| 引用本文: | 吴茵,任炳楠,张淼,隋峰.黄连配方颗粒化学成分的体内代谢研究[J].中国现代应用药学,2022,39(2):147-153. |
| WU Yin,REN Bingnan,ZHANG Miao,SUI Feng.Study on Metabolism of Chemical Constituents in Coptidis Rhizome Dispensing Granule in Vivo[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(2):147-153. |
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| 摘要: |
| 目的 探讨黄连配方颗粒主要成分体内代谢过程。方法 采用UHPLC-TOF/MS技术，根据精确相对分子质量和碎片离子分析，并结合相关文献检索及质谱裂解规律，比较黄连配方颗粒给药前后大鼠血清及尿液的图谱。结果 在黄连给药组血清中共发现原型成分15个，代谢物9个；尿液中原型成分18个，代谢物20个。结论 本研究较为全面地阐释了黄连配方颗粒主要化学成分的体内过程，研究结果可为黄连配方颗粒药动学、代谢产物推测、药效物质基础及作用机制等研究提供参考依据。 |
| 关键词: 黄连配方颗粒 体内代谢 UHPLC-Q-TOF/MS |
| DOI：10.13748/j.cnki.issn1007-7693.2022.02.002 |
| 分类号:R917 |
| 基金项目:国家自然科学基金项目(81903819)；中国中医科学院科技创新工程(CI2021A03803)；河北省自然基金项目(H2019307100)；第64批中国博士后科学基金(2018M641609)；河北省中医药管理局科研计划项目(2018071) |
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| Study on Metabolism of Chemical Constituents in Coptidis Rhizome Dispensing Granule in Vivo |
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WU Yin1,2, REN Bingnan2, ZHANG Miao1, SUI Feng1
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1.Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing 100700, China;2.Hebei General Hospital, Shijiazhuang 050051, China
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| Abstract: |
| OBJECTIVE To explore the metabolism process of major chemical constituents in Coptidis Rhizome Dispensing Granule. METHODS UHPLC-TOF/MS technology was adopted. Based on accurate relative molecular mass and fragment ion analysis, combined with relevant literature search and mass spectrometry fragmentation rules, the chromatograms of serum and urine of rats were compared before and after administration of Coptidis Rhizome Dispensing Granule. RESULTS Fifteen parent components and 9 metabolites were found in containing drug serum. There were 18 parent components and 20 metabolites in containing drug urine. CONCLUSION This study comprehensively explains the in vivo process of the main chemical components of Coptidis Rhizome Dispensing Granule. The results can provide scientific reference for the study of pharmacokinetics, metabolite prediction, pharmacodynamic material basis and mechanism of action about Coptidis Rhizome Dispensing Granule. |
| Key words: Coptidis Rhizome Dispensing Granule metabolism in vivo UHPLC-Q-TOF/MS |
