糙叶败酱总环烯醚萜苷的纯化工艺及抗炎活性研究

Study on Purification Process and Anti-inflammatory Activity of Total Iridoid Glycosides from Patrinia Scabra Bunge

关键词:  糙叶败酱  总环烯醚萜苷  大孔树脂  纯化工艺  抗炎作用

Key words:  Patrinia scabra Bunge  total iridoid glycosides  macroporous resin  purification process  anti-inflammatory

摘要:

目的 优选大孔树脂富集纯化糙叶败酱总环烯醚萜苷的工艺条件并研究其抗炎活性。方法 以紫外分光光度法测得的总环烯醚萜苷含量为指标，通过静态、动态吸附-解吸附能力考察，筛选适合纯化糙叶败酱总环烯醚萜苷的大孔树脂，进而通过考察上样浓度、最佳上样量、乙醇体积分数、洗脱剂用量、洗脱流速等优选其富集纯化工艺。采用二甲苯致小鼠耳肿胀试验，以阿司匹林为阳性对照药，对糙叶败酱总环烯醚萜苷的抗炎活性进行评价。结果 AB-8大孔树脂为纯化糙叶败酱总环烯醚萜苷的最适材料，最佳纯化工艺条件：上样量4 BV、浓度为1.10 mg·mL^-1，以2 BV·h^-1的流速进行动态吸附，加4 BV水洗脱除杂，然后用4 BV的50%乙醇以2 BV·h^-1的流速进行解吸。在此条件下，总环烯醚萜苷含量由纯化前的8.27%提高到纯化后的35.92%，约为纯化前的4.4倍。纯化后的总环烯醚萜苷的高剂量组（200 mg·kg^-1）能有效降低二甲苯诱发的小鼠耳肿胀，其抑制率达53.20%，较空白对照组具有显著性差异。结论 优选得到的以AB-8大孔树脂为填料优化的纯化工艺可行；糙叶败酱总环烯醚萜苷具有一定的抗炎作用。

Abstract:

OBJECTIVE To optimize the technological conditions for the enrichment and purification of total iridoid glycosides from Patrinia scabra Bunge by macroporous resin and study its anti-inflammatory activity. METHODS The content of total iridoid glycosides was determined through the ultraviolet spectrophotometry and was taken as an index. The macroporous resin which was suitable for purifying the total iridoid glycosides from Patrinia scabra Bunge was screened through the static and dynamic adsorption-desorption ability. Furthermore, the enrichment and purification process of Patrinia scabra was optimized by investigating the sample concentration, optimum sample volume, ethanol volume fraction, elution dosage, elution flow rate and so on. The anti-inflammatory activity of the total iridoid glycosides from Patrinia scabra Bunge was evaluated by the xylene-induced ear swelling test with the aspirin as positive control group. RESULTS AB-8 macroporous resin was the optimal material for the purification of total iridoid glycosides from Patrinia scabra Bunge. The optimum purification conditions were as follows:the dosage of sample volume was 4 BV, the concentration was 1.10 mg·mL^-1, the dynamic adsorption was carried out at the flow rate of 2 BV·h^-1, the impurity was washed out with 4 BV water, and then the desorption was carried out with 50% ethanol of 4 BV at the flow rate of 2 BV·h^-1. Under these conditions, the content of total iridoid glycosides increased from 8.27% to 35.92% after purification, which was about 4.4 times of that before purification. The high-dose group of total iridoid glycosides(200 mg·kg^-1) could effectively reduce the xylene-induced ear swelling in mice, and the inhibition rate reached 53.20%, which was significantly compared with the blank control group. CONCLUSION The optimized purification process with AB-8 macroporous resin as filler is feasible, and the total iridoid glycosides of Patrinia scabra Bunge possess certain anti-inflammatory effect.