夹竹桃花脂溶性成分提取工艺优化及其生物活性分析

Optimization of Extraction Technology and Biological Activities of Liposoluble Components from Nerium indicum Mill. Flower

关键词:  夹竹桃花  脂溶性成分  气相色谱质谱联用仪  抗氧化活性  抑菌活性

Key words:  Nerium indicum Mill. flower  liposoluble components  GC-MS  antioxidant activity  antibacterial activity

摘要:

目的 研究夹竹桃花脂溶性成分的提取工艺、组成成分及生物活性。方法 利用Box-Behnken响应面法优化提取工艺，GC-MS分析组成成分，滤纸片扩散法和二倍连续梯度稀释法分析抑菌活性，并测定抗氧化活性。结果 最佳提取工艺为索氏提取4 h，连续提取4次，料液比1∶ 15，得率9.16%，比优化前提高了13.09%。脂溶性成分含有烷烃类、醛类和酯类等30种化合物，具有还原能力和清除DPPH自由基、羟自由基、超氧自由基的能力，对枯草芽孢杆菌、金黄色葡萄球菌、大肠埃希菌和铜绿假单胞菌的最小抑菌浓度分别为1.22，1.72，0.75，1.96 g·L^–1，最小杀菌浓度分别为2.34，2.84，1.88，3.32 g·L^–1。结论 脂溶性成分最佳提取工艺得率为9.16%，以烷烃类、醛类、酯类为主，具有一定的抗氧化和抑菌活性，可为夹竹桃资源的开发利用提供参考。

Abstract:

OBJECTIVE To study the extraction process, composition and biological activity of liposoluble components from Nerium indicum Mill. flower. METHODS Box-Behnken response surface methodology was used to optimize the extraction technology of liposoluble components, and the chemical compositions were studied by GC-MS. The antibacterial activity was determined by paper diffusion method and double continuous gradient dilution method, while study the antioxidant activity. RESULTS The results of optimum extraction condition were as follows:Extraction duration 4 h, 4 times of extraction, and the solid-liquid ratio 1:15, the actual value of the yield was 9.16%, which increased by 13.09% compared with the initial extraction conditions. There were 30 compounds including alkanes, aldehydes and esters in liposoluble components. The liposoluble components had the reduction ability and scavenging ability of DPPH radicals, hydroxyl radicals and superoxide anion radicals. The minimum inhibitory concentration of liposoluble components on Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were 1.22, 1.72, 0.75, 1.96 g·L^–1, respectively. The minimum bactericidal concentration were 2.34, 2.84, 1.88, 3.32 g·L^–1, respectively. CONCLUSION Actual value of optimized extraction technology is 9.16%, the composition of liposoluble components from Nerium indicum Mill. flower mainly include alkanes, aldehydes and esters, and have certain antioxidant activity and antibacterial activity, which can provide reference for the development and utilization of Nerium indicum Mill. resources.