畲药树参茎化学成分分析及抗炎活性部位高效液相指纹图谱的建立

Analysis Chemical Constituents in the Stems of She Medicine Dendropanax Dentiger and Establishing HPLC Fingerprint of Its Anti-inflammatory Active Fraction

关键词:  树参茎  抗炎  活性部位  高效液相色谱法  指纹图谱

Key words:  stems of Dendropanax dentiger  anti-inflammatory  active fraction  HPLC  fingerprint

摘要:

目的 对畲药树参茎所含化学成分进行确证分析，并建立抗炎活性部位的高效液相色谱指纹图谱。方法 采用液相色谱-质谱联用、核磁共振波谱仪等分析仪器分离鉴定树参茎中的化学成分；采用高效液相色谱法建立抗炎活性部位指纹图谱：色谱柱为Waters Sunfire^TM C₁₈(4.6 mm×250 mm，5 μm)，流动相为乙腈-混合水溶液(含甲酸0.2%和四氢呋喃0.2%)，梯度洗脱。检测波长为256 nm，柱温30 ℃，体积流量为1.0 mL·mim^–1，分析时间为80 min，进样量为5 μL。结果 从树参茎中共分离鉴定出10个化合物，分别是绿原酸(1)、3,4-O-二咖啡酰基奎宁酸(2)、3,5-O-二咖啡酰基奎宁酸(3)、4,5-O-二咖啡酰基奎宁酸(4)、saikolignanoside A(5)、芦丁(6)、山柰酚-3-O-芸香糖苷(7)、紫丁香苷(8)、松柏醇(9)、芥子醛葡萄糖苷(10)；12批样品抗炎活性部位指纹图谱相似度为0.901~0.992，均符合要求。结论 树参茎中主要含有酚酸类、黄酮类、苯丙素类化合物；建立的指纹图谱不仅能体现树参茎抗炎的化学物质基础，而且鉴别方法简便、重复性好，可为树参茎的品质评价及主要抗炎活性成分的确证提供科学依据。

Abstract:

OBJECTIVE To confirm and analyze the chemical constituents in the stems of Dendropanax dentiger,and establish the HPLC fingerprint of its anti-inflammatory active fraction. METHODS Compounds were isolated and identified by LC-MS,NMR and other analysis instruments.HPLC with Waters Sunfire^TM C₁₈ column (4.6 mm×250 mm,5 μm) was used.Mobile phase consisted acetonitrile-aqueous solution including 0.2% formic acid and 0.2% tetrahydrofuran with gradient elution.Detection wavelength was 256 nm.Column temperature was 30℃.Flow rate was 1.0 mL·mim^–1 and analytic time was 80 min.Injection volume was 5 μL.RESULTS Ten compounds were isolated and identified as chlorogenic acid (1),3,4-O-caffeoylquinic acid methyl ester (2),3,5-O-caffeoylquinic acid methyl ester (3),4,5-O-caffeoylquinic acid methyl ester (4),saikolignanoside A (5),rutin (6),kaempferol-3-O-rutinoside (7),syringin (8),coniferol (9),sinapaldehyde glucoside (10).Under the same fingerprint chromatogram condition,the methodological results were satisfactory to 12 batches of the anti-inflammatory active fractions.And the similarity range was 0.901-0.992.CONCLUSION The stems of Dendropanax dentiger mainly contain phenolic acids,flavonoids and phenylpropanoids.The established fingerprint can not only reflect the chemical basis of anti-inflammatory of the She medicine,but the identification method also is simple and reproducible.It can provide a scientific basis for the quality evaluation and the confirmation of main anti-inflammatory active components of the stems of Dendropanax dentiger.