一测多评法同时测定不同产地墨旱莲中9种成分及其质量评价研究

Study on Simultaneous Determination of 9 Components in Eclipta Herba from Different Growing Areas by QAMS Method and It’s Quality Evaluation

关键词:  墨旱莲  产地  一测多评法  灰色关联分析  质量评价

Key words:  Ecliptae Herba  growing areas  quantitative analysis of multi-components by single-marker  grey correlation analysis  quality evaluation

摘要:

目的 建立一测多评法同时测定墨旱莲中9种成分，并对不同产地墨旱莲质量进行综合评价。方法 采用HPLC，使用Agilent ZORBAX Eclipse Plus C₁₈色谱柱(250 mm×4.6 mm，5 μm)，以乙腈-0.2%磷酸水溶液为流动相，梯度洗脱，流速1 mL·min^–1，进样量20 μL，柱温维持在25 ℃，检测波长210，349 nm。以蟛蜞菊内酯为内参物，计算其他8个成分的相对校正因子，并计算含量，同时采用外标法测定墨旱莲中9种成分的含量，比较一测多评法计算值与外标法实测值的差异。采用灰色关联度法构建墨旱莲质量的灰色关联质量评价模型。结果 以蟛蜞菊内酯为内参物测得的其他成分相对校正因子系统适应性良好，一测多评法得到的结果与实测值之间无显著性差异。不同产地墨旱莲相对关联度范围为0.440~0.747，说明各产地墨旱莲的质量存在差异。结论 建立的一测多评法适合于墨旱莲多成分含量测定，基于灰色关联度模型可以更客观地评价不同产地墨旱莲的质量。

Abstract:

OBJECTIVE To establish a quantitative analysis of multi-components by single-marker (QAMS) method for the simultaneous content determination of 9 components in Ecliptae Herba,and to comprehensively evaluate the quality of Ecliptae Herba from different growing areas.METHODS An Agilent ZORBAX Eclipse Plus C₁₈ column (250 mm×4.6 mm,5 μm) was used in the HPLC assay.Acetonitrile-0.2% phosphoric acid aqueous solution with gradient elution was employed as the mobile phase.The flow rate was 1 mL·min^-1,the in jection volume was 20 μL,the column temperature was maintained at 25℃ and the detection wavelength was 210,349 nm.Wedelolactone was used as the internal reference substance to calculate the relative correction factors of the other eight constituents and calculate their contents accordingly.Moreover,the external standard method was used to quantify the 9 components in Ecliptae Herba.Comparison of the difference between the QAMS and external standard method was made.The grey correlation quality evaluation model of Ecliptae Herba was established by grey correlation analysis.RESULTS The relative correction factors of other components measured with wedelolactone as internal reference had good system adaptability,and there was no significant difference between the results obtained by QAMS and the measured values.The relative correlation degree of Ecliptae Herba from different growing areas ranged from 0.440 to 0.747,indicating that there were differences in the quality of Ecliptae Herba from different growing areas.CONCLUSION The established QAMS method is suitable for the content determination of multi index components of Ecliptae Herba.Based on the grey correlation model,the quality of Ecliptae Herba from different growing areas can be evaluated more objectively.