Box-Behnken响应面法优化毛蕊花糖苷离心萃取工艺

Optimization of Centrifugal Extraction Technology of Verbascoside by Box-Behnken Response Surface Methodology

关键词:  毛蕊花糖苷  响应面法  Box-Behnken设计  离心萃取工艺

Key words:  verbascoside  response surface methodology  Box-Behnken design  technology of centrifugal extraction

摘要:

目的 采用离心萃取系统萃取毛蕊花糖苷提取物。方法 通过单因素试验，研究油相中毛蕊花糖苷含量、流量、油水相比、萃取次数、转速、pH对毛蕊花糖苷萃取率的影响。利用响应面Box-Behnken实验设计探究油相中毛蕊花糖苷含量、流量、转速、油水相比对毛蕊花糖苷萃取率的影响并优化最优工艺参数。结果 单因素试验结果表明：萃取最佳条件为油相中毛蕊花糖苷含量为19.20 mg·mL^-1，转速为48 Hz，流量为300 mL·min^-1，油相与水相比=1∶1，乙酸加入量为2%，萃取次数为3次。通过响应面分析发现各因素对毛蕊花糖苷萃取率的影响顺序为油相中毛蕊花糖苷含量>油水相比>流量>转速；在最佳条件下：油相中毛蕊花糖苷含量21.50 mg·mL^-1、转速48 Hz、流量290 mL·min^-1、油水相比1∶1，3次平行试验得到的毛蕊花糖苷萃取率分别达82.49%，79.38%，81.76%，与预测值80.03％的相对误差分别为3.07%，0.81%，2.16%。结论 此方法能够有效对毛蕊花糖苷进行纯化，为毛蕊花糖苷的开发利用提供理论依据。

Abstract:

OBJECTIVE To extract the extract of verbascoside by centrifugal extraction system. METHODS The effects of content of verbascoside in oil phase, flow rate, oil-water ratio, extraction times, rotation speed, pH on the extraction rate of verbascoside were studied by single factor experiment. Box-Behnken response surface design was used to explore the effects of the content of verbascoside in oil phase, flow rate, rotation speed, oil-water ratio on the extraction rate of verbascoside and optimize the optimal process parameters. RESULTS The results of single factor experiment showed that the optimum extraction conditions were as follows: the content of verbascoside in oil phase was 19.20 mg·mL^-1, the rotation speed was 48 Hz, the flow rate was 300 mL·min^-1, the ratio of oil phase to water phase was 1∶1, the amount of acetic acid was 2%, and the extraction times were 3 times. Through response surface analysis, it was found that the order of factors affecting the extraction rate of verbascoside was as follows: content of verbascoside in oil phase>oil/water phase ratio>flow rate>rotation speed. Under the optimum conditions, the content of verbascoside in oil phase was 21.50 mg·mL^-1, the rotating speed was 48 Hz, and the flow rate was 290 mL·min^-1, with oil/water ratio of 1∶1, the results of three parallel experiments showed that the extraction rates of verbascoside were 82.49%, 79.38% and 81.76%, respectively, and the relative errors compared with the predicted value of 80.03% were 3.07%, 0.81% and 2.16%, respectively. CONCLUSION This method can effectively purify verbascoside and provide theoretical basis for the development and utilization of verbascoside.