地奥心血康治疗大鼠非酒精性单纯性脂肪肝的实验研究

Experimental Study on Nonalcoholic Fatty Liver in Rats Treated with Di’ao Xinxuekang

关键词:  地奥心血康  非酒精性单纯性脂肪肝  脂质代谢紊乱  肝损伤  实验研究

Key words:  Di'ao Xinxuekang  nonalcoholic fatty liver  lipid metabolism disorder  liver injury  experimental study

摘要:

目的 研究地奥心血康对大鼠非酒精性单纯性脂肪肝的治疗作用。方法 将SD大鼠(♂)随机分为正常组和造模组,高脂高胆固醇饲料饲喂造模组大鼠8周,待造模成功后将造模组大鼠随机分为模型组、阿托伐他汀组(2.0 mg·kg^–1),地奥心血康高、中、低(100,30,10 mg·kg^–1)剂量组,每组10只,灌胃给药连续8周。油酸-棕榈酸(oleic acid-palmitic acid,OA-PA)刺激HepG2细胞建立脂质蓄积模型,阿托伐他汀和地奥心血康干预。测定血脂、肝脂质、肝功能、肝脏指数;测定细胞脂质和转氨酶水平;HE染色、透射电镜、油红O染色观察肝脏与肝细胞超微结构形态学变化及脂质沉积情况。结果 与正常组比较,模型组大鼠肝脏指数、血脂、肝脂质及肝脏病理评分显著升高,肝功能异常,HepG2细胞脂质蓄积、转氨酶升高;与模型组比较,地奥心血康组及阿托伐他汀组肝脏指数和转氨酶降低,脂质代谢和脂质蓄积明显改善,肝细胞脂肪变性明显减轻(P<0.05或P<0.01)。结论 地奥心血康可有效治疗大鼠非酒精性单纯性脂肪肝。

Abstract:

OBJECTIVE To study the therapeutic effect of Di'ao Xinxuekang on nonalcoholic fatty liver in rats.METHODS Male SD rats were randomly divided into normal group and model group. Rats in model group were fed with high-fat and high cholesterol diet for 8 weeks. After the model was successfully established, the model rats were randomly divided into model group, atorvastatin group(2.0 mg·kg^-1) and Di'ao Xinxuekang high, medium and low(100, 30, 10 mg·kg^-1)dose groups, ten rats in each group. They were administered by gavage for 8 weeks. Oleic acid-palmitic acid(OA-PA) stimulated HepG2 cells to establish a lipid accumulation model, which was intervened by atorvastatin and Di'ao Xinxuekang. Blood lipid,liver lipid, liver function and liver index were measured. The levels of lipid and transaminase were measured. The ultrastructural changes and lipid deposition of liver and hepatocytes were observed by HE staining, transmission electron microscope and oil red O staining.RESULTS Compared with normal group, in the model group, the liver index, blood lipid, liver lipid and liver pathological score increased significantly, liver dysfunction and the lipid accumulation and transaminase of HepG2 cells increased.Compared with model group, Di'ao Xinxuekang group and atorvastatin group decreased liver index and transaminase, significantly improved lipid metabolism and lipid accumulation, and significantly reduced hepatocyte steatosis(P<0.05 or P<0.01).CONCLUSION Di'ao Xinxuekang can effectively treat nonalcoholic fatty liver in rats.