离子色谱法测定小儿止咳糖浆中氯化铵的含量

Determination of Ammonium Chloride in Xiao'er Zhike Syrup by Ion Chromatography

关键词:  离子色谱法  小儿止咳糖浆  氯化铵  氯离子

Key words:  ion chromatography  Xiao'er Zhike syrup  ammonium chloride  chloride

摘要:

目的 建立离子色谱法测定小儿止咳糖浆中氯化铵含量的方法。方法 离子色谱法采用IonPac^TM AS11-HC阴离子交换色谱柱(2.0 mm×250 mm)与IonPac^TM AG11-HC保护柱(2.0 mm×50 mm)，检测器为抑制电导检测器，电导检测池温度为35℃，柱温为30℃，进样量为25 μL，以10 mmol·L^–1氢氧化钾溶液为流动相，流速为0.5 mL·min^–1。结果 氯离子浓度为10~400μg·mL^–1时，峰面积与其浓度呈良好的线性关系(r=1.000 0，n=6);平均回收率为100.57%(RSD=0.41%，n=6)。结论 该方法专属性强、准确度高，可用于小儿止咳糖浆中氯化铵的含量测定。

Abstract:

OBJECTIVE To establish an ion chromatography method for determination of ammonium chloride in Xiao'er Zhike syrup. METHODS The chromatographic separation was performed on an IonPac^TMAS11-HC anion exchange column (2.0 mm×250 mm) and IonPac^TM AG11-HC guard column(2.0 mm×50 mm), using suppressed conductivity detector, the temperature of the detection tank and the column were set at 35℃ and 30℃ respectively, the injection volume was 25 μL, an isocratic eluent of 10 mmol·L^-1 KOH was chosen as mobile phase and the flow rate was 0.5 mL·min^-1. RESULTS A good linear relationship between the peak area and its concentration was observed within the range of 10-400 μg·mL^-1(r=1.000 0, n=6); The average recovery was 100.57%(RSD=0.41%, n=6).CONCLUSION The method is specific and accurate, and can be used for determination of ammonium chloride in Xiao'er Zhike syrup.