UPLC-Q-TOF-MS分析罗汉果叶醇提物中化学成分

Analysis of Chemical Constituents in Methanol Extracts of Siraitia Grosvenorii Leaves by UPLC-Q-TOF-MS

关键词:  UPLC-Q-TOF-MS  罗汉果叶  醇提物  化学成分

Key words:  UPLC-Q-TOF-MS  Siraitia grosvenorii leaves  ethanol extract  chemical constituents

摘要:

目的 建立超高效液相色谱-四极杆串联飞行时间质谱(ultra performance liquidchromatography-quadrupole time-of-flight mass spectrometry，UPLC-Q-TOF-MS)对罗汉果叶醇提取物溶液进行快速定性的方法。方法 色谱条件：色谱柱为ACQUITY UPLC BEH C₁₈(2.1 mm×100 mm，1.8 μm)；进样体积为5 μL；流速为0.3 mL·min^-1，流动相为0.1%甲酸水溶液(A)-乙腈溶液(B)进行梯度洗脱；质谱条件：电喷雾离子源(ESI)，检测范围m/z 50~1 000，分别采用正、负离子进行MSE模式采集，基于高分辨分子量和质谱碎片信息，结合数据库匹配对质谱数据进行分析。结果 从罗汉果叶醇提取物中共表征出64个化合物，其中黄酮类7个，酸类14个，酯类12个，三萜类10个，酮类4个，醇类4个以及其他类13个。结论 UPLC-Q-TOF-MS技术为罗汉果叶中化学成分的分析提供了简便、高效、精确的参考方法。

Abstract:

OBJECTIVE To establish a rapid qualitative method for ethanol extract from the leaves of Siraitia grosvenorii by using ultra performance liquidchromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS). METHODS Chromatographic column: ACQUITYUPLC UPLC BEH C₁₈(2.1 mm×100 mm, 1.8 μm); injection volume: 5 μL; flow rate: 0.3 mL·min^-1, mobile phase was 0.1% formic acid solution(A)-acetonitrile solution(B) with gradient elution sequence; mass spectrum condition: electrospray ion source(ESI), detection range m/z 50-1 000, positive and negative ions were used for MSE mode acquisition respectively, based on the high-resolution molecular weight and mass spectrum fragment information, combined with database matching, the mass spectrum data were analyzed. RESULTS A total of 64 compounds were characterized from the alcohol extract of Siraitia grosvenorii leaves, including seven flavonoids, fourteen acids, twelve esters, ten triterpenoids, four ketones, four alcohols and thirteen others. CONCLUSION UPLC-Q-TOF-MS provides a simple, efficient and accurate method for the identification of chemical constituents in the alcohol extraction of Siraitia grosvenorii leaves.