银柴胡超临界CO2萃取工艺优化及其萃取物成分分析

Optimization of Supercritical CO2 Extraction Process and Component Analysis of Extract from Stellariae Radix

关键词:  银柴胡  超临界CO₂萃取  Box-Behnken响应面法  脂质  UHPLC-Q-Exactive Orbitrap MS

Key words:  Stellariae Radix  supercritical CO₂ extraction  Box-Behnken response surface methodology  lipids  UHPLC-Q-Exactive Orbitrap MS

摘要:

目的 优化银柴胡脂质成分超临界CO₂萃取工艺并分析萃取物成分。方法 采用Box-Behnken响应面法优化银柴胡脂质成分超临界CO₂萃取工艺，确定最佳工艺参数，并应用UHPLC-Q-Exactive Orbitrap MS对萃取物进行分析。结果 经优化后最佳萃取条件：萃取压力35 MPa，萃取温度47 ℃，萃取时间3 h，在此条件下萃取得率为(0.332±1.31)%。鉴定分析结果显示银柴胡脂质成分含有甘油磷脂、鞘脂、甘油酯、固醇类、孕烯醇酮脂、脂肪酰、糖脂7大类，29亚类，2 542个脂质分子，其中固醇类相对含量最高为88.29%，并首次发现银柴胡中含有植物甾醇酯、酵母甾醇及神经酰胺类成分。结论 优化后的萃取工艺和成分分析结果可为银柴胡药效物质研究及其进一步开发利用奠定基础。

Abstract:

OBJECTIVE To optimize the supercritical CO₂ extraction process to extract lipid of Stellariae Radix and analyze its components.METHODS The supercritical CO₂ extraction process of lipid of Stellariae Radix was optimized by Box-Behnken response surface methodology,and the optimal process parameters were determined.The lipid component was analyzed by UHPLC-Q-Exactive Orbitrap MS.RESULTS The best extraction process of lipid of Stellariae Radix was extraction pressure 35 MPa,extraction temperature 47℃,extraction time 3 h.Under this condition,the extraction yield of lipid was (0.332±1.31)%.Identification analysis results showed that the lipid component of Stellariae Radix contained 7 categories,including glycerophospho lipids,sphingolipids,glycerolipids,sterol lipids,pregnenolone lipids,fatty acyls,and glycolipids,29 subcategories,and 2 542 lipid species.The relative content of sterollipids was up to 88.29% of the lipid.Furthermore,it was first discovered phytosterol esters,yeast sterols,and ceramides from Stellariae Radix.CONCLUSION The optimized extraction process and analysis of lipid components lay the foundation for the research and further development of Stellariae Radix medicinal substances.