富氢盐水对高原环境下烧伤小鼠肠道损伤的影响

Effects of Hydrogen-rich Saline on Intestinal Damage in High-altitude Burned Mice

关键词:  富氢盐水  烧伤  高原环境  肠道损伤  氧化应激  炎症反应

Key words:  hydrogen-rich saline  burns  high altitude environment  intestinal damage  oxidative stress  inflammatory response

摘要:

目的 探究富氢盐水对高原环境下烧伤小鼠肠道损伤的保护作用及可能机制。方法 36只♀BALB/c小鼠随机分为假烧伤组、烧伤模型组和富氢盐水组,实验均在低压氧舱室中模拟海拔3000m的高原环境。假烧伤组小鼠背部采用温水浴(37℃,10s)模拟致伤,烧伤模型组和富氢盐水组小鼠背部采用沸水浴法(90℃,10s)致30%TBSA Ⅲ烫伤。烧伤后,富氢盐水组注射富氢盐水10mL·kg^-1(0.6mmol·L^-1),假烧伤组和烧伤模型组注射等量生理盐水。通过分析小肠组织病理情况,血清中脂多糖的表达及肠道中紧密黏连蛋白1(zonula occludens-1,ZO-1)、闭合蛋白(Occludin)、胆碱乙酰转移酶(choline acetyltransferase,ChAT)、酪氨酸羟化酶(tyrosine hydroxylase,TH)和神经元型一氧化氮合酶(neuronal nitric oxide synthase,nNOS)的表达以阐明富氢盐水对高原环境下烧伤小鼠肠道屏障及肠神经系统的影响。另外,通过分析血清中丙二醛(malondialdehyde,MDA)、过氧化氢酶(catalase,CAT)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)的表达,肠道中炎症因子TNF-α、IL-1β和IL-17A的表达及CD3 T淋巴细胞浸润情况以探究富氢盐水对高原烧伤小鼠氧化应激及炎症反应的影响。结果 与假烧伤组相比,烧伤模型组小鼠表现出明显的肠道病理损伤、ZO-1、Occludin、ChAT、TH和nNOS的表达显著降低;富氢盐水显著改善了模型组小鼠的肠道病理损伤、肠黏膜屏障损伤,并且对肠神经系统表现出积极作用。另外,富氢盐水可显著调控烧伤模型组小鼠血清中MDA、CAT及GSH-Px的表达,降低炎症细胞因子的表达,减少CD3 T淋巴细胞的浸润。结论 富氢盐水可减轻高原环境下烧伤小鼠的肠道病理损伤,修复肠黏膜屏障,并对肠神经系统发挥保护作用,其作用可能通过调控氧化应激及炎症反应实现。

Abstract:

OBJECTIVE To explore the protective effect and possible mechanism of hydrogen-rich saline on intestinal injury in burned mice under high altitude environment.METHODS Thirty-six female BALB/c mice were randomly divided into sham group, burn model group and hydrogen-rich saline group, all experiments were conducted in a low-pressure oxygen chamber to simulate an altitude of 3000 m. The back of mice in the sham group was simulated with a warm water bath(37 ℃,10 s), and the back of mice in the burn model group and the hydrogen-rich saline group was simulated with a boiling water bath(90 ℃, 10 s) to cause 30% TBSA Ⅲ scald. After burn, mice in the hydrogen-rich saline group was injected with10 mL·kg^-1(0.6 mmol·L^-1), while the sham group and the burn model group were injected with the same amount of saline. The effects of hydrogen-rich saline on the intestinal barrier and intestinal nervous system of burned mice were analyzed by analyzing the pathological of intestinal tissue, the expression of lipopolysaccharide in serum and the expression of zonula occludens-1(ZO-1), Occludin, choline acetyltransferase(ChAT), tyrosine hydroxylase(TH) and neuronal nitric oxide synthase(nNOS) in intestinal. In addition, by analyzing the expression of malondialdehyde(MDA), catalase(CAT) and glutathione peroxidase(GSH-Px) in serum, expression of inflammatory factors of TNF-α, IL-1β and IL-17A and CD3 T lymphocyte infiltration in intestinal, the effects of hydrogen-rich saline on oxidative stress and inflammatory response in mice burned at high altitude were investigated.RESULTS Compared with the sham group, mice in the burn model group showed significant intestinal pathological damage, and the expressions of ZO-1, Occludin, ChAT, TH and nNOS were significantly decreased.Hydrogen-rich saline significantly improved intestinal pathological injury and intestinal mucosal barrier injury in the model group, and also showed a positive effect on the intestinal nervous system. In addition, hydrogen-rich saline could significantly regulate the expressions of MDA, CAT and GSH-Px in serum, reduce the expression of inflammatory cytokines, and reduce the infiltration of CD3 T lymphocytes in the intestinal tissue of burn model mice.CONCLUSION Hydrogen-rich saline can relieve the intestinal pathological damage, repair the intestinal mucosal barrier, and protect the intestinal nervous system of burned mice under high altitude environment, which may be realized by regulating oxidative stress and inflammatory response.