| 引用本文: | 肖小武,许妍,刘静佳,李丹,姜军华,罗跃华,洪挺.UHPLC-MS/MS同时测定参桂鹿茸丸中5种人参皂苷的含量[J].中国现代应用药学,2023,40(8):1104-1109. |
| XIAO Xiaowu,XUYan,LIU Jingjia,LI Dan,JIANG Junhua,Luo Yuehua,HONG Ting.Simultaneous determination of 5 ginsenosides in Shengui Lurong Pills by UHPLC-MS/MS[J].Chin J Mod Appl Pharm(中国现代应用药学),2023,40(8):1104-1109. |
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| UHPLC-MS/MS同时测定参桂鹿茸丸中5种人参皂苷的含量 |
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肖小武1, 许妍1, 刘静佳2, 李丹1, 姜军华1, 罗跃华1, 洪挺1
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1.江西省药品检验检测研究院, 江西省药品与医疗器械质量工程技术研究中心, 国家药品监督管理局中成药质量评价重点实验室, 南昌 330029;2.南昌理工学院, 南昌 330044
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| 摘要: |
| 目的 建立同时测定参桂鹿茸丸中5种人参皂苷含量的UHPLC-MS/MS方法。方法 选用Agilent C18色谱柱(100 mm×2.1 mm，1.8 μm)，柱温25 ℃，以甲醇-0.1%甲酸水梯度洗脱，流速为0.3 mL·min–1，ESI离子源，采用正离子多反应监测(MRM)监测模式。结果 人参皂苷Re、Rg1、Rf、Rb1以及拟人参皂苷F11浓度范围内线性良好，r2均>0.999 6，加样回收率为96.3%~104.9%，RSD(n=9)均<3.0%，仪器精密度、稳定性以及重复性的RSD均<3.0%。结论 该方法简单，准确，重复性好，可用于参桂鹿茸丸的质量控制和评价。 |
| 关键词: 参桂鹿茸丸 人参皂苷 超高液相色谱-串联质谱法 |
| DOI：10.13748/j.cnki.issn1007-7693.20220741 |
| 分类号:R284.1 |
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| Simultaneous determination of 5 ginsenosides in Shengui Lurong Pills by UHPLC-MS/MS |
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XIAO Xiaowu1, XUYan1, LIU Jingjia2, LI Dan1, JIANG Junhua1, Luo Yuehua1, HONG Ting1
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1.Jiangxi Institute For Drug Control, Jiangxi Provincial Engineering Research Center for Drug and Medical Device Quality, NMPA Key Laboratory for Quality Evaluation of Traditional Chinese Medicine, Nanchang 330029, China;2.Nanchang Institute of Technology, Nanchang 330044, China
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| Abstract: |
| OBJECTIVE To establish a UHPLC-MS/MS method for simultaneous determination of five ginsenosides in Shengui lurong pills. METHODS The determination was performed on Agilent C18(100 mm×2.1 mm, 1.8 μm) column with column temperature of 25 ℃. Methanol and 0.1% aqueous formic acid solution were used as mobile phase for gradient elution with the flow rate of 0.3 mL·min-1. Ion source was electrospray ion source, positive ion mode monitoring was conducted and MRM mode was adopted. RESULTS The linear ranges of ginsenoside Re, Rg1, Rf, Rb1 and pseudo-ginsenoside F11 had good resolution and linear relationships(r2≥0.999 6). The spiked recoveries were in the range of 96.3%-104.9% with RSD(n=9)<3.0%. RSDs of instrument precision, stability and reproducibility tests were <3.0%. CONCLUSION The method is simple, accurate, reliable, and can be uesd for quality control and evaluation of Shengui Lurong pills. |
| Key words: Shengui Lurong pills ginsenoside UHPLC-MS/MS |
