孕康口服液对化学损伤联合米非司酮致气血两虚型流产模型大鼠的保胎作用研究

Study on Tocolysis Effect of Yunkang Oral Liquid on Chemical Injury Combined with Mifepristone Induced Abortion Model Rats with Deficiency of Qi and Blood

关键词:  孕康口服液  先兆流产  激素  气血两虚

Key words:  Yunkang oral liquid  threatened abortion  hormones  Qi and blood deficiency

摘要:

目的 探究孕康口服液对限饲联合乙酰苯肼、环磷酰胺及米非司酮致气血两虚型流产模型大鼠的保胎作用。方法 将妊娠大鼠随机分为正常对照组、模型对照组、黄体酮组及孕康口服液低、中、高剂量组(4，6，9 mL·kg^–1)，每组12只。前9 d，一边造模一边给药。第10，11天，除正常对照组外，其余各组均灌胃米非司酮。给药期间检测自主活动、肛温、体质量，血液分析仪检测红细胞数(red blood cells，RBC)、血红蛋白数(hemoglobin，HGB)、白细胞数(white blood cells，WBC)。第12天处死大鼠后解剖计算胚胎丢失率、子宫、卵巢、胸腺和肝脏系数，酶联免疫法检测血清中激素β-人绒毛膜促性腺激素(β-human chorionic gonadotropin，β-HCG)、孕酮(progesterone，P)、雌二醇(estradiol，E₂)水平及肿瘤坏死因子(tumor necrosis factor，TNF-α)、γ干扰素(γ levels of interferon，IFN-γ)、白细胞介素4(interleukin 4，IL-4)、白细胞介素10(interleukin 10，IL-10)的水平，免疫组化法检测子宫组织中孕激素受体(progesterone receptor，PR)及血管内皮生长因子(vascular endothelial growth factor，VEGF)表达。结果 与模型对照组比较，不同剂量孕康口服液组大鼠体质量、肛温、自主活动次数显著增加，血液中RBC、HGB和WBC数目增多，子宫、卵巢、胸腺和肝脏系数显著升高，血清中P、E₂、IL-4、IL-10水平显著增加(P<0.05或P<0.01)，胚胎丢失率明显下降，血清中TNF-α、IFN-γ水平降低(P <0.05或P<0.01)，子宫内膜的病变程度减轻，子宫组织PR和VEGF蛋白表达明显上调。结论 孕康口服液对乙酰苯肼+环磷酰胺+限饲联合米非司酮造成气血两虚型流产模型大鼠具有明显的保胎作用。

Abstract:

OBJECTIVE To study the tocolysis effect of Yunkang oral liquid on the abortion model rats with Qi and blood deficiency induced by feeding restriction combined with acetophenazine, cyclophosphamide and mifepristone. METHODS The pregnant rats were randomly divided into normal control group, model control group, progesterone group and Yunkang oral liquid low, middle and high dose groups(4, 6, 9 mL·kg^-1), with 12 rats in each group. In the first 9 d, the drug was administered while building the model. On the 10th and 11th day, except for the normal control group, all the other groups were given mifepristone by gavage. The following indicators were tested during the administration period: autonomous activities, rectal temperature, weight, and the number of red blood cells(RBC), hemoglobin(HGB), white blood cells(WBC) were detected by the blood analyzer. Rats were sacrificed on the 12th day, and the embryo loss rate, uterus, ovary, thymus and liver coefficients were calculated by dissection, enzyme-linked immunosorbent assay was used to detect serum levels of hormones β-human chorionic gonadotropin(β-HCG), progesterone(P), estradiol(E₂) level and tumor necrosis factor(TNF-α), γ levels of interferon(IFN-γ), interleukin 4(IL-4), and interleukin 10(IL-10), the expressions of progesterone receptor(PR) and vascular endothelial growth factor(VEGF) in uterine tissue were detected by immunohistochemistry. RESULTS Compared with the model control group, the body weight, rectal temperature and the number of autonomous activities of the rats in different doses of Yunkang oral liquid group increased significantly, and the number of RBC, HGB and WBC in the blood increased, uterine, ovary, thymus and liver coefficients were significantly increased, the levels of P, E₂, IL-4 and IL-10 in serum were significantly increased(P<0.05 or P<0.01), the embryo loss rate was significantly decreased, and the levels of TNF-α and IFN-γ in serum were decreased(P<0.05 or P<0.01), the degree of endometrial lesions was reduced, and the expressions of PR and VEGF proteins in uterine tissue were significantly up-regulated. CONCLUSION It shows that Yunkang oral liquid has obvious effect on tocolysis in rats with Qi and blood deficiency caused by acetophenhydrazine+cyclophosphamide+restricted feeding combined with mifepristone.