2019-nCoV灭活方式对酶免疫法检测急性淋巴细胞白血病患儿血清甲氨蝶呤浓度的影响

Effect of 2019-nCoV Inactivation Method on Serum Methotrexate Concentration in Children with Acute Lymphoblastic Leukemia Detected by Enzyme Immunoassay

关键词:  2019-nCoV  紫外线灭活  恒温水浴灭活  甲氨蝶呤

Key words:  2019-nCoV  ultraviolet-inactivated  heat-inactivated  methotrexate

摘要:

目的 研究紫外线和加热2种2019-nCoV灭活方法对酶免疫法检测甲氨蝶呤(methotrexate，MTX)浓度的影响，探讨该方法在疫情防控期间的应用。方法 将临床送检采血管以75%乙醇表面消毒，低速离心后，将血清分为未灭活组、紫外线灭活组和加热灭活组。其中紫外线灭活组将MTX质控液及血清样本于二级生物安全柜密闭后，紫外灯照射30 min灭活；加热灭活组将质控液与血清样本置于密封袋中，于56℃恒温水槽中水浴30 min灭活。结果 紫外线灭活组和加热灭活组MTX检测结果与未灭活组相比，差异均无统计学意义。未灭活组、紫外线灭活组和加热灭活组3组91%~95.5%的浓度点分布在Bland-Altman图中的95%一致性区间内。未灭活组、紫外线灭活组和加热灭活组3组相关系数均>0.98(P<0.001)，表明3组结果一致性良好。结论 以紫外线照射30 min或56℃加热灭活30 min处理对酶免疫法检测MTX浓度检测无影响，可用于疫情防控期间急性淋巴细胞白血病患儿MTX酶免疫法治疗药物监测的开展。

Abstract:

OBJECTIVE To study the effects of ultraviolet and heating inactivation of 2019-nCoV on the detection of methotrexate(MTX) concentration by enzyme immunoassay, then to explore the application of this method in epidemic prevention and control. METHODS The blood collection tubes sent to clinical inspection were surface-sterilized with 75% ethanol. After low-speed centrifugation, the serums were divided into non-inactivated, ultraviolet-inactivated and heat-inactivated groups. Then, the ultraviolet-inactivated group was prepared by irradiating the MTX quality control solution and serum samples in a secondary biological safety cabinet under ultraviolet lamp for 30 min. The inactivation method for the heat- inactivation group was heating the quality control solution and serum samples in a seal bag under water bath at 56℃ for 30 min. RESULTS Compared with non-inactivation group, there were no significant difference in MTX detection results of both ultraviolet inactivation and heat-inactivation groups. The results of 91%-95.5% concentration point distribution of the non-inactivated group, ultraviolet-inactivated group and heat-inactivated group were within the 95% concordance interval of the Bland-Altman plot. The correlation coefficients of the non-inactivation group, ultraviolet inactivation group and heat-inactivation group were all >0.98(P<0.001), indicating that the results of the three groups had good consistency. CONCLUSION The inactivation of blood samples in blood vessels by ultraviolet ray for 30 min or heating at 56℃ for 30 min have no effect on the detection of MTX concentration by enzyme immunoassay, which can be used to monitor the concentration of MTX for children with acute lymphoblastic leukemia during the epidemic prevention and control.