绿梅花中4种成分双标多测法的建立

Establishment of Two Reference Substances for Determination of Multiple Components for 4 Components in Armeniaca Mume

关键词:  双标多测法  双标线性校正法  绿梅花  DRS Origin软件

Key words:  two reference substances for determination of multiple components  double standard linear correction method  Armeniaca mume  DRS Origin software

摘要:

目的 建立双标多测法同时测定绿梅花中绿原酸、芦丁、金丝桃苷、异槲皮苷4种成分含量，更经济高效地控制和评价绿梅花质量。方法 采用高效液相色谱法，流动相以含0.1%甲酸的乙腈溶液为A相，0.1%甲酸溶液为B相，流速1.0 mL·min^-1，检测波长355 nm，柱温25 ℃。以DRS Origin软件筛选出绿原酸和金丝桃苷为参照物，采用双标线性校正法预测色谱峰的保留时间，以相对校正因子法测定各成分含量。结果 双标线性校正法预测不同成分保留时间的预测结果准确且优于相对保留时间法；相对校正因子法计算的含量与外标法的结果相对误差均<1％，无明显差异。10批绿梅花中绿原酸、芦丁、金丝桃苷、异槲皮苷的含量分别为46.18~69.68，3.91~8.00，1.76~3.11，3.21~5.56 mg·g^-1。结论 建立的双标多测法预测待测成分保留时间的结果准确，色谱柱的适用范围广，节省对照品，经济高效；同时也可准确测定各成分含量，为绿梅花多成分质量控制和评价提供新的思路和方法。

Abstract:

OBJECTIVE To establish two reference substances for determination of multiple components method to simultaneously determine the content of 4 components of chlorogenic acid, rutin, hyperoside, and isoquercitrin in Armeniaca mume to control and evaluate the quality of Armeniaca mume cost-effectively and efficiently. METHODS HPLC was used. The mobile phase was phase A with 0.1% formic acid in acetonitrile and phase B with 0.1% formic acid solution. The flow rate was 1.0 mL·min^-1, the detection wavelength was 355 nm, and the column temperature was 25 ℃. Chlorogenic acid and hypericin were screened out by DRS Origin software as reference substances, the retention time of chromatographic peaks was predicted by double-standard linear calibration method, and the content of each component was determined by relative correction factor method. RESULTS The prediction results of the double-standard linear correction method in predicting the retention time of different components were accurate and better than the relative retention time method; the relative error between the content calculated by the relative correction factor method and the results of the external standard method was < 1%, and there was no significant difference. The contents of chlorogenic acid, rutin, hyperoside and isoquercitrin in 10 batches of Armeniaca mume were 46.18-69.68, 3.91-8.00, 1.76-3.11, 3.21-5.56 mg·g^-1, respectively. CONCLUSION The established two reference substances for determination of multiple components method is accurate in predicting the retention time of the components to be tested, the chromatographic column has a wide range of applications, saves reference substances, and is economical and efficient; at the same time, the content of each component can be accurately determined, which provides new ideas and methods for the quality control and evaluation of Armeniaca mume.