胆汁酸-肠道菌群轴在2型糖尿病中的作用

Role of Bile Acid-gut Microbiota Axis in Type 2 Diabetes Mellitus

关键词:  胆汁酸  肠道菌群  2型糖尿病

Key words:  bile acid  gut microbiota  type 2 diabetes mellitus

摘要:

目的 分析2型糖尿病(type 2 diabetes mellitus,T2DM)患者和正常人群胆汁酸与肠道菌群差异,探讨胆汁酸-肠道菌群轴在T2DM中的作用。方法 利用代谢组学、16S rRNA测序手段分别对T2DM患者中的血清胆汁酸含量及粪便肠道微生物进行检测及分析,结合斯皮尔曼相关性分析,明确胆汁酸-肠道菌群在T2DM中的代谢对话关系。结果 血清胆汁酸含量和肠道微生物的丰度在T2DM患者与正常人群中存在一定的差异。与正常人群相比,甘氨熊脱氧胆酸、牛磺鹅脱氧胆酸、甘氨鹅脱氧胆酸的含量在T2DM患者中显著降低;T2DM患者中肺炎克雷伯菌属、普拉梭菌属的相对丰度较正常人群明显升高,而狄氏副拟杆菌属、普雷沃菌属、艾克曼菌属、双歧杆菌属的相对丰度明显降低;斯皮尔曼相关性分析表明甘氨熊脱氧胆酸与狄氏副拟杆菌属、艾克曼菌属呈正相关,与克雷伯氏菌属呈负相关。结论 胆汁酸-肠道菌群轴是维持机体稳态的必要因素,在T2DM中发挥重要作用。

Abstract:

OBJECTIVE To analyze the difference of bile acid and gut microbiota between type 2 diabetes mellitus(T2DM) patients and normal people, and explore the role of bile acid-gut microbiota axis in T2DM. METHODS The contents of serum bile acid and faecal and gut microbiota in T2DM patients were detected and analyzed by using metabonomics and 16S rRNA sequencing methods respectively. And then the study combined with Spearman correlation analysis, to clarify the metabolic dialogue between bile acid and gut microbiota in T2DM. RESULTS The content of serum bile acid and the abundance of gut microbiota in T2DM patients were different from that in normal people. Compared with normal people, the levels of glycoursodeoxycholic acid, taurochenodeoxycholic acid and glycochenodeoxycholic acid in T2DM patients were significantly lower; the relative abundance of Klebsiella and Plasmodium in T2DM patients was significantly higher and the relative abundance of Parabacteroides, Prevotella,Akkermansia and Bifidobacterium were significantly lower than normal people. Spearman correlation analysis showed that glycoursodeoxycholic acid was positively correlated with the abundance of Parabacteroides and Akkermansia and negatively correlated with the abundance of Klebsiella. CONCLUSION Bile acid-gut microbiota axis is a necessary factor to maintain the body's homeostasis and plays an important role in T2DM.